Use of human α-satellite deoxyribonucleic acid to detect Y-specific centromeric sequences

Sandra P.T. Tho, Ali Behzadian, J. Rogers Byrd, Paul G. McDonough

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

The Y alphoid deoxyribonucleic acid probe Y97 has proved to be specific for the human Y centromere and to define a Y-specific 5.5 kb Eco RI fragment. Three experiments were designed to evaluate the sensitivity and the specificity of this Y alphoid probe Y97. In the first experiment the centromeric Y-specific 5.5 kb Eco RI fragment was clearly seen in the mixture of 0.050 μg of male DNA with 4.950 μg of female DNA (1%). In the second experiment the same dilutional study was applied to the Yq11-related probe 4B-2 for comparison purpose. In the third experiment, hybridization with the Y97 probe was performed on 20 subjects with mosaic cell lines containing a cytogenetically identifiable Y (n = 10) and a cytogenetically unidentifiable minute (n = 10) fragment. Nineteen of the 20 study subjects demonstrated the Y-specific 5.5 kb Eco RI hybridization band with the centromeric Y97 probe. These experiments demonstrated the utility of the Y97 probe to consistently identify cytogenetically altered Y chromosome fragments and confirm the mapping of the alphoid repeat sequences to the centromeric region of the Y chromosome.

Original languageEnglish (US)
Pages (from-to)1553-1557
Number of pages5
JournalAmerican journal of obstetrics and gynecology
Volume159
Issue number6
DOIs
StatePublished - Dec 1988

Keywords

  • 4B-2
  • Y chromosome
  • Y97
  • deoxyribonucleic acid probes

ASJC Scopus subject areas

  • Obstetrics and Gynecology

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