Use of intracytoplasmic sperm injection (ICSI) to generate transgenic animals

Stefan Moisyadi, Joseph Michael Kaminski, Ryuzo Yanagimachi

Research output: Contribution to journalReview article

26 Citations (Scopus)

Abstract

Even though intracytoplasmic sperm injection (ICSI) has been widely used for the production of offspring in human infertility clinics and in reproductive research laboratories using mice, many researchers engaged in animal transgenesis still consider it somewhat cumbersome. The greatest advantage of ICSI-mediated transgenesis is that it allows introduction of very large DNA transgenes (e.g., yeast artificial chromosomes), with relatively high efficiency into the genomes of hosts, as compared to pronuclear injection. Recently, we have developed an active form of intracytoplasmic sperm injection-mediated transgenesis (ICSI-Tr) with fresh sperm utilizing transposons. The transgenic efficiencies rival all transgenic techniques except that of lentiviral methods.

Original languageEnglish (US)
Pages (from-to)47-60
Number of pages14
JournalComparative Immunology, Microbiology and Infectious Diseases
Volume32
Issue number2
DOIs
StatePublished - Mar 1 2009

Fingerprint

intracytoplasmic sperm injection
Gene Transfer Techniques
transgenic animals
Genetically Modified Animals
Intracytoplasmic Sperm Injections
yeast artificial chromosomes
human fertility
genetically modified organisms
Yeast Artificial Chromosomes
Transgenes
transposons
Infertility
transgenes
Spermatozoa
researchers
spermatozoa
Research Personnel
Genome
injection
Injections

Keywords

  • Transgenesis
  • Transposase
  • Transposon
  • piggyBac

ASJC Scopus subject areas

  • Microbiology
  • Immunology and Allergy
  • Immunology
  • veterinary(all)
  • Infectious Diseases

Cite this

Use of intracytoplasmic sperm injection (ICSI) to generate transgenic animals. / Moisyadi, Stefan; Kaminski, Joseph Michael; Yanagimachi, Ryuzo.

In: Comparative Immunology, Microbiology and Infectious Diseases, Vol. 32, No. 2, 01.03.2009, p. 47-60.

Research output: Contribution to journalReview article

Moisyadi, Stefan ; Kaminski, Joseph Michael ; Yanagimachi, Ryuzo. / Use of intracytoplasmic sperm injection (ICSI) to generate transgenic animals. In: Comparative Immunology, Microbiology and Infectious Diseases. 2009 ; Vol. 32, No. 2. pp. 47-60.
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