One of the antibody families utilized by the A/J mouse in its response to p-azophenylarsonate (Ars) is characterized by the expression of the major anti-arsonate cross-reactive idiotype (CRI) of the A strain. This family has been termed the Ars-A family. A hybridoma antibody (HP 101F11) obtained after immunization of an A/J mouse with Ars was identified initially as displaying the CRI, but was subsequently found to bind antigen at a level much lower than most members of the Ars-A family. The results of binding studies suggested that HP 101F11 possesses reduced avidity for antigen. When isolated light and heavy chains were allowed to recombine with the heavy and light chains of a strongly antigen-binding, strongly CRI-positive antibody of the Ars-A family (HP 93G7), the low level of antigen binding by HP 101F11 was found to be due to a structurally variant heavy chain. Whereas antibodies of the Ars-A family with normal avidity for antigen had been shown to use the J(H)2 joining segment gene, amino acid sequence analysis of HP 101F11 revealed that this antibody has a J(H) segment with a sequence identical to that encoded by a portion of a different J(H) gene, J(H)4. The implication that 101F11 uses the J(H)4 gene instead of J(H)2 was supported by the observation that the productively rearranged gene is associated with an Eco R1 restriction fragment 0.95 Kb smaller than the corresponding fragments of Ars-A hybridomas with normal avidity for antigen. The size difference of 0.95 Kb corresponds exactly to the known distance between the J(H)2 and J(H)4 genes in BALB/c germline DNA. In addition to the structural differences immediately attributable to the use of J(H)4, HP 101F11 has shown an amino acid interchange in the D(H) segment, and a single amino acid deletion at the D(H)-J(H) boundary. These results show that variation among members of the Ars-A family in the D(H) and/or J(H) segments provides alternative structural forms of Ars-A antibodies upon which selective processes can operate during the course of an immune response.
|Original language||English (US)|
|Number of pages||8|
|Journal||Journal of Immunology|
|State||Published - 1984|
ASJC Scopus subject areas
- Immunology and Allergy