Use of single (4B-2) and repetitive copy (pS4) deoxyribonucleic acid (DNA) probes to characterize translocated Y DNA in a pedigree with recurrent abortion

S. P.T. Tho, J. A. Tischfield, Paul G McDonough

Research output: Contribution to journalArticle

Abstract

Probes for unique and repetitive copy deoxyribonucleic acid (DNA) are available to detect and characterize Y DNA. The probe pS4 detects repetitive copy DNA mapped to Yq12. The upper limits of the pS4 sequences are defined by the upper limits of C-banding. The probe 4B-2 is a recombinant phage construct developed from a Y library and contains a unique copy 3.3 kb Eco RI fragment mapped to Yq11. A family was ascertained through a pregnant female who had a history of four consecutive abortions and two normal daughters. Cytogenetic analysis revealed the mother and one of her daughters to have a 46,XX,15p+ karyotype. Amniocytes were karyotyped as 46,XY,15p+. Genomic DNA from controls, mother, daughters, and amniocytes was digested with Mbo I and hybridized to 32P-labeled pS4 probe. DNAs from both 46,XX,15p+ females and 46,XY,15p+ amniocytes demonstrated a clear male-specific 2.3 kb band. Digestion of the same genomic DNAs with Eco RI and blot hybridization to 32P-labeled 4B-2 probe revealed the 3.3 kb male-specific band only in the 46,XY,15p+ amniocyte DNA. The additional sequences located on 15p segregating in the female members of this family correspond to Yq12. The effect of this additional DNA on gametogenesis is unknown.

Original languageEnglish (US)
Pages (from-to)428-432
Number of pages5
JournalFertility and Sterility
Volume48
Issue number3
DOIs
StatePublished - Jan 1 1987

Fingerprint

Habitual Abortion
Pedigree
DNA
Nuclear Family
Deoxyribonuclease EcoRI
Mothers
Gametogenesis
Cytogenetic Analysis
Karyotype
Bacteriophages
Libraries
Digestion

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

Cite this

Use of single (4B-2) and repetitive copy (pS4) deoxyribonucleic acid (DNA) probes to characterize translocated Y DNA in a pedigree with recurrent abortion. / Tho, S. P.T.; Tischfield, J. A.; McDonough, Paul G.

In: Fertility and Sterility, Vol. 48, No. 3, 01.01.1987, p. 428-432.

Research output: Contribution to journalArticle

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