Use of standardized and conventional allergen extracts in prick skin testing

B. J. Lavins, William K. Dolen, Harold S. Nelson, Richard W. Weber

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

This study examined whether commercially available conventional and standardized allergen extracts differ enough in potency to affect routine prick skin test results. Extracts of white oak, timothy, Bermuda, Russian thistle, short ragweed, sagebrush, Altemaria, and cat dander were examined in allergic patients and in nonatopic subjects with no personal or family history of asthma, rhinitis, or eczema. Conventional nonstandardized extracts (1:10 or 1:20 wt/vol) from two sources were compared with three concentrations (100,000, 10,000, and 1000 AU/ml) of a single standardized extract. Preparations were compared in the allergic patients with computerized planimetry, and in all patients and subjects with a conventional skin test grading system. Skin test area for the conventional extracts generally fell between the 10,000 and 100,000 AU/ml concentrations of the standardized extract. Skin test reactivity to at least one allergen extract occurred in 31% of the nonatopic subjects; there was no difference between the number of 3+ and 4+ reactions for conventional and standardized extracts. Results indicate that standardized and conventional extracts are frequently similar, but are not directly interchangeable.

Original languageEnglish (US)
Pages (from-to)658-666
Number of pages9
JournalThe Journal of Allergy and Clinical Immunology
Volume89
Issue number3
DOIs
StatePublished - Mar 1992

Fingerprint

Skin Tests
Allergens
Skin
Salsola
Bermuda
Dander
Ambrosia
Artemisia
Eczema
Rhinitis
Cats
Asthma

Keywords

  • Allergen extracts
  • atopy
  • skin testing
  • standardization

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Use of standardized and conventional allergen extracts in prick skin testing. / Lavins, B. J.; Dolen, William K.; Nelson, Harold S.; Weber, Richard W.

In: The Journal of Allergy and Clinical Immunology, Vol. 89, No. 3, 03.1992, p. 658-666.

Research output: Contribution to journalArticle

Lavins, B. J. ; Dolen, William K. ; Nelson, Harold S. ; Weber, Richard W. / Use of standardized and conventional allergen extracts in prick skin testing. In: The Journal of Allergy and Clinical Immunology. 1992 ; Vol. 89, No. 3. pp. 658-666.
@article{a07115d8b8b343ec8add7231be98e2c4,
title = "Use of standardized and conventional allergen extracts in prick skin testing",
abstract = "This study examined whether commercially available conventional and standardized allergen extracts differ enough in potency to affect routine prick skin test results. Extracts of white oak, timothy, Bermuda, Russian thistle, short ragweed, sagebrush, Altemaria, and cat dander were examined in allergic patients and in nonatopic subjects with no personal or family history of asthma, rhinitis, or eczema. Conventional nonstandardized extracts (1:10 or 1:20 wt/vol) from two sources were compared with three concentrations (100,000, 10,000, and 1000 AU/ml) of a single standardized extract. Preparations were compared in the allergic patients with computerized planimetry, and in all patients and subjects with a conventional skin test grading system. Skin test area for the conventional extracts generally fell between the 10,000 and 100,000 AU/ml concentrations of the standardized extract. Skin test reactivity to at least one allergen extract occurred in 31{\%} of the nonatopic subjects; there was no difference between the number of 3+ and 4+ reactions for conventional and standardized extracts. Results indicate that standardized and conventional extracts are frequently similar, but are not directly interchangeable.",
keywords = "Allergen extracts, atopy, skin testing, standardization",
author = "Lavins, {B. J.} and Dolen, {William K.} and Nelson, {Harold S.} and Weber, {Richard W.}",
year = "1992",
month = "3",
doi = "10.1016/0091-6749(92)90371-8",
language = "English (US)",
volume = "89",
pages = "658--666",
journal = "Journal of Allergy and Clinical Immunology",
issn = "0091-6749",
publisher = "Mosby Inc.",
number = "3",

}

TY - JOUR

T1 - Use of standardized and conventional allergen extracts in prick skin testing

AU - Lavins, B. J.

AU - Dolen, William K.

AU - Nelson, Harold S.

AU - Weber, Richard W.

PY - 1992/3

Y1 - 1992/3

N2 - This study examined whether commercially available conventional and standardized allergen extracts differ enough in potency to affect routine prick skin test results. Extracts of white oak, timothy, Bermuda, Russian thistle, short ragweed, sagebrush, Altemaria, and cat dander were examined in allergic patients and in nonatopic subjects with no personal or family history of asthma, rhinitis, or eczema. Conventional nonstandardized extracts (1:10 or 1:20 wt/vol) from two sources were compared with three concentrations (100,000, 10,000, and 1000 AU/ml) of a single standardized extract. Preparations were compared in the allergic patients with computerized planimetry, and in all patients and subjects with a conventional skin test grading system. Skin test area for the conventional extracts generally fell between the 10,000 and 100,000 AU/ml concentrations of the standardized extract. Skin test reactivity to at least one allergen extract occurred in 31% of the nonatopic subjects; there was no difference between the number of 3+ and 4+ reactions for conventional and standardized extracts. Results indicate that standardized and conventional extracts are frequently similar, but are not directly interchangeable.

AB - This study examined whether commercially available conventional and standardized allergen extracts differ enough in potency to affect routine prick skin test results. Extracts of white oak, timothy, Bermuda, Russian thistle, short ragweed, sagebrush, Altemaria, and cat dander were examined in allergic patients and in nonatopic subjects with no personal or family history of asthma, rhinitis, or eczema. Conventional nonstandardized extracts (1:10 or 1:20 wt/vol) from two sources were compared with three concentrations (100,000, 10,000, and 1000 AU/ml) of a single standardized extract. Preparations were compared in the allergic patients with computerized planimetry, and in all patients and subjects with a conventional skin test grading system. Skin test area for the conventional extracts generally fell between the 10,000 and 100,000 AU/ml concentrations of the standardized extract. Skin test reactivity to at least one allergen extract occurred in 31% of the nonatopic subjects; there was no difference between the number of 3+ and 4+ reactions for conventional and standardized extracts. Results indicate that standardized and conventional extracts are frequently similar, but are not directly interchangeable.

KW - Allergen extracts

KW - atopy

KW - skin testing

KW - standardization

UR - http://www.scopus.com/inward/record.url?scp=0026578959&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026578959&partnerID=8YFLogxK

U2 - 10.1016/0091-6749(92)90371-8

DO - 10.1016/0091-6749(92)90371-8

M3 - Article

C2 - 1545087

AN - SCOPUS:0026578959

VL - 89

SP - 658

EP - 666

JO - Journal of Allergy and Clinical Immunology

JF - Journal of Allergy and Clinical Immunology

SN - 0091-6749

IS - 3

ER -