Usefulness of CD11a and CD18 in flow cytometric immunophenotypic analysis for diagnosis of acute promyelocytic leukemia

Yi Zhou, Jeffrey L. Jorgensen, Sa A. Wang, Farhad Ravandi, Jorge Cortes, Hagop M. Kantarjian, L. Jeffrey Medeiros, Sergej Konoplev

Research output: Contribution to journalArticle

Abstract

Acute promyelocytic leukemia (APL) is an aggressive disease that requires prompt diagnosis and therapy. Flow cytometry immunophenotyping can serve as a screening test for APL before the results of cytogenetic or molecular testing for t(15;17)(q22;q21)/PML-RAR a are available. CD11a and CD18 are leukocyte β2-integrin subunits expressed in most acute myeloid leukemia (AML) types, but are often dimly expressed or absent in APL. We used flow cytometry immunophenotyping with an antibody panel including CD11a and CD18 to assess 36 APL and 33 other AML cases. HLA-DR, CD11a, and CD18 were absent in 81% of APL and 12% of other AML cases (specificity, 88%). By further including combinations of HLA-DR-, CD2+, and either CD11a- or CD18-, we identified 92% of APL cases with 85% specificity. These data compare favorably with the combination of HLA-DR- , CD34-, and CD117+ for APL diagnosis, which had a sensitivity of 64% in this study.

Original languageEnglish (US)
Pages (from-to)744-750
Number of pages7
JournalAmerican Journal of Clinical Pathology
Volume138
Issue number5
DOIs
StatePublished - Nov 1 2012
Externally publishedYes

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Acute Promyelocytic Leukemia
HLA-DR Antigens
Acute Myeloid Leukemia
Immunophenotyping
Flow Cytometry
Integrins
Cytogenetics
Leukocytes
Antibodies

Keywords

  • β2-integrin
  • Acute progranulocytic leukemia
  • CD11a
  • CD18
  • Flow cytometry

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Usefulness of CD11a and CD18 in flow cytometric immunophenotypic analysis for diagnosis of acute promyelocytic leukemia. / Zhou, Yi; Jorgensen, Jeffrey L.; Wang, Sa A.; Ravandi, Farhad; Cortes, Jorge; Kantarjian, Hagop M.; Medeiros, L. Jeffrey; Konoplev, Sergej.

In: American Journal of Clinical Pathology, Vol. 138, No. 5, 01.11.2012, p. 744-750.

Research output: Contribution to journalArticle

Zhou, Yi ; Jorgensen, Jeffrey L. ; Wang, Sa A. ; Ravandi, Farhad ; Cortes, Jorge ; Kantarjian, Hagop M. ; Medeiros, L. Jeffrey ; Konoplev, Sergej. / Usefulness of CD11a and CD18 in flow cytometric immunophenotypic analysis for diagnosis of acute promyelocytic leukemia. In: American Journal of Clinical Pathology. 2012 ; Vol. 138, No. 5. pp. 744-750.
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