Usefulness of HPLC methodology for the characterization of combinations of the common β chain variants HBS s, c, and o-ARAB, and the α chain variant HB g-philadelphia

Ferdane Kutlar, Abdullah Kutlar, E. Nuguid, J. Prchal, T. H J Huisman

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We have analyzed the blood samples from seven members of two families that contained mixtures of different hemoglobin types due to the simultaneous presence of different β chain variants and the α chain variant Hb G-Philadelphia. The methods used were isoelectrofocusing and cation exchange high performance liquid chromatography for the separation of the hemoglobin components, and reversed phase high performance liquid chromatography for that of the various α and β chains; the chromatographic procedures also provided quantitative data. Application of these fast and simple high performance liquid chromatographic methods greatly aided in defining the different hemoglobinopathies that were present in these persons, including an additional αthalassemia-2 heterozygosity or homozygosity.

Original languageEnglish (US)
Pages (from-to)55-66
Number of pages12
JournalHemoglobin
Volume17
Issue number1
DOIs
StatePublished - Jan 1 1993

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High performance liquid chromatography
Hemoglobins
High Pressure Liquid Chromatography
Cations
Hemoglobinopathies
Ion exchange
Blood
Thalassemia
Reverse-Phase Chromatography
Liquids
hemoglobin G Philadelphia

ASJC Scopus subject areas

  • Hematology
  • Genetics(clinical)
  • Clinical Biochemistry
  • Biochemistry, medical
  • Biochemistry

Cite this

Usefulness of HPLC methodology for the characterization of combinations of the common β chain variants HBS s, c, and o-ARAB, and the α chain variant HB g-philadelphia. / Kutlar, Ferdane; Kutlar, Abdullah; Nuguid, E.; Prchal, J.; Huisman, T. H J.

In: Hemoglobin, Vol. 17, No. 1, 01.01.1993, p. 55-66.

Research output: Contribution to journalArticle

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