Utilization of precursor essential fatty acids in culture by skin fibroblasts from schizophrenic patients and normal controls

S. P. Mahadik, Nitin Sudhakar Shendarkar, R. E. Scheffer, S. Mukherjee, E. E. Correnti

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Based on the lower levels of long-chain polyunsaturated analogs of essential fatty acids (EPUFAs) in plasma membrane phospholipids of red blood cells, brain and cultured skin fibroblasts from schizophrenic patients, a defective utilization (uptake, conversion to EPUFAs and incorporation into membrane phospholipids) of precursor EFAs has been suggested. Utilization of radiolabeled linoleic (LA, 18:2(n-6)) and alpha-linoleic (ALA, 18:3(n-3)) acids was studied in cultured skin fibroblasts from patients with established schizophrenia and at the first episode of psychosis, and normal controls. Uptake and incorporation of both the EFAs were similar in fibroblasts from both groups of patients studied compared with normal controls. However, although the utilization of LA into arachidonic acid (AA, 20:4n-6) was similar in patients and controls, the utilization of eicosapentaenoic acid (EPA, 20:5(n-3)) into docosahexaenoic acid (DHA, 22:6(n-3)) was significantly lower in first-episode psychotic patients (patients, 96.33 ± 27.16 versus normals, 161.66 ± 26.33 nmoles per mg total protein; P = < 0.001). This data indicates that the level of Δ6- as well as Δ5-desaturase may be normal. However, the levels of Δ4-desaturase may be lower in fibroblasts from schizophrenic patients even at the first episode of psychosis.

Original languageEnglish (US)
Pages (from-to)65-70
Number of pages6
JournalProstaglandins Leukotrienes and Essential Fatty Acids
Volume55
Issue number1-2
DOIs
StatePublished - Jan 1 1996

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Essential Fatty Acids
Fibroblasts
Cell culture
Skin
Phospholipids
Eicosapentaenoic Acid
Docosahexaenoic Acids
Psychotic Disorders
Cell membranes
Arachidonic Acid
Brain
Blood
Cells
Membranes
Acids
Schizophrenia
Erythrocytes
Cell Membrane
Proteins

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology

Cite this

Utilization of precursor essential fatty acids in culture by skin fibroblasts from schizophrenic patients and normal controls. / Mahadik, S. P.; Shendarkar, Nitin Sudhakar; Scheffer, R. E.; Mukherjee, S.; Correnti, E. E.

In: Prostaglandins Leukotrienes and Essential Fatty Acids, Vol. 55, No. 1-2, 01.01.1996, p. 65-70.

Research output: Contribution to journalArticle

Mahadik, S. P. ; Shendarkar, Nitin Sudhakar ; Scheffer, R. E. ; Mukherjee, S. ; Correnti, E. E. / Utilization of precursor essential fatty acids in culture by skin fibroblasts from schizophrenic patients and normal controls. In: Prostaglandins Leukotrienes and Essential Fatty Acids. 1996 ; Vol. 55, No. 1-2. pp. 65-70.
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