v-rel Induces ectopic expression of an adhesion molecule, DM-GRASP, during B-lymphoma development

G. Zhang, Clive A. Slaughter, E. H. Humphries

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

In an effort to identify aberrantly expressed genes in v-rel-induced tumors, monoclonal antibodies were developed that reacted selectively with avian B-cell tumors. One antibody, HY78, immunoprecipitated a 120-kDa glycoprotein (p120) from cells that express v-rel. N-terminal amino acid sequencing of p120 identified a 27-amino-acid sequence that is also present in DM-GRASP, an adhesion molecule belonging to the immunoglobulin superfamily. Evidence from tissue distribution, immunological cross-reaction, PCR amplification, cDNA cloning, and DNA sequence shows that p120 is indeed DM-GRASP. Northern (RNA) analysis using a probe from the DM-GRASP gene identified a 5.3-kb transcript in mRNA from bursa, thymus, and brain as well as from v-rel-induced B-cell lymphomas but not from bursal B cells. The induction of this protein by v-rel during the development of bursal B-cell lymphomas appears, therefore, to be ectopic in nature. Overexpression of v- rel or c-rel in chicken embryonic fibroblasts, B-cell lines, and spleen mononuclear cells induces the expression of DM-GRASP. The ratio of DM-GRASP to v-Rel was fivefold higher than that of DM-GRASP/c-Rel in a B-cell line, DT95. Interestingly, the presence of HY78 antibody inhibits the in vitro proliferation of v-rel-transformed cells but not cells that immortalized by myc. These data suggest that DM-GRASP is one of the genes induced during v- rel-mediated tumor development and that DM-GRASP may be involved in the growth of v-rel tumor cells.

Original languageEnglish (US)
Pages (from-to)1806-1816
Number of pages11
JournalMolecular and Cellular Biology
Volume15
Issue number3
StatePublished - Jan 1 1995
Externally publishedYes

Fingerprint

Lymphoma
B-Lymphocytes
B-Cell Lymphoma
Oncogene Proteins v-rel
rel Genes
Neoplasm Antibodies
Cell Line
Neoplasms
Antibodies
Cross Reactions
Protein Sequence Analysis
Tissue Distribution
Thymus Gland
Genes
Immunoglobulins
Organism Cloning
Amino Acid Sequence
Chickens
Glycoproteins
Spleen

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

v-rel Induces ectopic expression of an adhesion molecule, DM-GRASP, during B-lymphoma development. / Zhang, G.; Slaughter, Clive A.; Humphries, E. H.

In: Molecular and Cellular Biology, Vol. 15, No. 3, 01.01.1995, p. 1806-1816.

Research output: Contribution to journalArticle

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abstract = "In an effort to identify aberrantly expressed genes in v-rel-induced tumors, monoclonal antibodies were developed that reacted selectively with avian B-cell tumors. One antibody, HY78, immunoprecipitated a 120-kDa glycoprotein (p120) from cells that express v-rel. N-terminal amino acid sequencing of p120 identified a 27-amino-acid sequence that is also present in DM-GRASP, an adhesion molecule belonging to the immunoglobulin superfamily. Evidence from tissue distribution, immunological cross-reaction, PCR amplification, cDNA cloning, and DNA sequence shows that p120 is indeed DM-GRASP. Northern (RNA) analysis using a probe from the DM-GRASP gene identified a 5.3-kb transcript in mRNA from bursa, thymus, and brain as well as from v-rel-induced B-cell lymphomas but not from bursal B cells. The induction of this protein by v-rel during the development of bursal B-cell lymphomas appears, therefore, to be ectopic in nature. Overexpression of v- rel or c-rel in chicken embryonic fibroblasts, B-cell lines, and spleen mononuclear cells induces the expression of DM-GRASP. The ratio of DM-GRASP to v-Rel was fivefold higher than that of DM-GRASP/c-Rel in a B-cell line, DT95. Interestingly, the presence of HY78 antibody inhibits the in vitro proliferation of v-rel-transformed cells but not cells that immortalized by myc. These data suggest that DM-GRASP is one of the genes induced during v- rel-mediated tumor development and that DM-GRASP may be involved in the growth of v-rel tumor cells.",
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