Vascular endothelial growth factor-stimulated actin reorganization and migration of endothelial cells is regulated via the serine/threonine kinase Akt

Manuel Morales-Ruiz, David J Fulton, Grzegorz Sowa, Lucia R. Languino, Yasushi Fujio, Kenneth Walsh, William C. Sessa

Research output: Contribution to journalArticle

320 Citations (Scopus)

Abstract

Vascular endothelial growth factor (VEGF) induces endothelial cell proliferation, migration, and actin reorganization, all necessary components of an angiogenic response. However, the distinct signal transduction mechanisms leading to each angiogenic phenotype are not known. In this study, we examined the ability of VEGF to stimulate cell migration and actin rearrangement in microvascular endothelial cells infected with adenoviruses encoding β-galactosidase (β-gal), activation-deficient Akt (AA-Akt), or constitutively active Akt (myr-Akt). VEGF increased cell migration in cells transduced with β-gal, whereas AA-Akt blocked VEGF-induced cell locomotion. Interestingly, myr-Akt transduction of bovine lung microvascular endothelial cells stimulated cytokinesis in the absence of VEGF, suggesting that constitutively active Akt, per se, can initiate the process of cell migration. Treatment of β-gal-infected endothelial cells with an inhibitor of NO synthesis blocked VEGF-induced migration but did not influence migration initiated by myr-Akt. In addition, VEGF stimulated remodeling of the actin cytoskeleton into stress fibers, a response abrogated by infection with dominant-negative Akt, whereas transduction with myr-Akt alone caused profound reorganization of F-actin. Collectively, these data demonstrate that Akt is critically involved in endothelial cell signal transduction mechanisms leading to migration and that the Akt/endothelial NO synthase pathway is necessary for VEGF-stimulated cell migration.

Original languageEnglish (US)
Pages (from-to)892-896
Number of pages5
JournalCirculation research
Volume86
Issue number8
DOIs
StatePublished - Apr 28 2000

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Protein-Serine-Threonine Kinases
Vascular Endothelial Growth Factor A
Actins
Endothelial Cells
Cell Movement
Signal Transduction
Galactosidases
Stress Fibers
Cytokinesis
Actin Cytoskeleton
Adenoviridae
Nitric Oxide Synthase
Cell Proliferation
Phenotype
Lung
Infection

Keywords

  • Actin
  • Angiogenesis
  • Cell migration
  • Nitric oxide
  • Vascular endothelial growth factor

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Vascular endothelial growth factor-stimulated actin reorganization and migration of endothelial cells is regulated via the serine/threonine kinase Akt. / Morales-Ruiz, Manuel; Fulton, David J; Sowa, Grzegorz; Languino, Lucia R.; Fujio, Yasushi; Walsh, Kenneth; Sessa, William C.

In: Circulation research, Vol. 86, No. 8, 28.04.2000, p. 892-896.

Research output: Contribution to journalArticle

Morales-Ruiz, Manuel ; Fulton, David J ; Sowa, Grzegorz ; Languino, Lucia R. ; Fujio, Yasushi ; Walsh, Kenneth ; Sessa, William C. / Vascular endothelial growth factor-stimulated actin reorganization and migration of endothelial cells is regulated via the serine/threonine kinase Akt. In: Circulation research. 2000 ; Vol. 86, No. 8. pp. 892-896.
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