Vascular endothelium viability and function after total cardiopulmonary bypass in neonatal piglets

Alain Serraf, Hassan Sellak, Philippe Hervé, Nicolas Bonnet, Monica Robotin, Hélène Detruit, Bruneau Baudet, Guy Michel Mazmanian, Claude Planche

Research output: Contribution to journalArticle

40 Scopus citations

Abstract

Endothelium dysfunction with severe pulmonary hypertension may occur after total cardiopulmonary bypass (CPB) in infants as a result of a widespread inflammatory response. The aim of this study was to separate out the effects of lung ischemia-reperfusion from membrane oxygenator-induced activation of leukocytes on the function and viability of the pulmonary and systemic endothelia in neonatal piglets submitted to 90-min total CPB followed by 60-min reperfusion or in sham animals. Hemodynamics, gas exchange, endothelial-dependent relaxation in pulmonary and femoral arteries, and lung and skeletal muscle myeloperoxidase activity were assessed before, during, and after CPB, i.e., after reperfusion. Pulmonary and aortic endothelial cells and circulating leukocytes were harvested to assess reperfusion-induced changes in endothelial cells' viability and proliferation, and leukocyte-endothelial cell adhesion and cytotoxicity. Gas exchange worsened after reperfusion with pulmonary hypertension, increase in lung but not skeletal myeloperoxidase, and reduction of endothelial-dependent relaxation in pulmonary but not femoral arteries. After reperfusion, viabilities of pulmonary and aortic endothelial cells were reduced to 50%, endothelial cell growths were faster in pulmonary arteries than aorta, and leukocyte-pulmonary endothelial cell adhesion and cytotoxicity increased. These results suggest that in total CPB lung ischemia-reperfusion aggravates the inflammatory response and predisposes the lung endothelium to leukocyte- mediated injury.

Original languageEnglish (US)
Pages (from-to)544-551
Number of pages8
JournalAmerican Journal of Respiratory and Critical Care Medicine
Volume159
Issue number2
DOIs
StatePublished - Jan 1 1999

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Critical Care and Intensive Care Medicine

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    Serraf, A., Sellak, H., Hervé, P., Bonnet, N., Robotin, M., Detruit, H., Baudet, B., Mazmanian, G. M., & Planche, C. (1999). Vascular endothelium viability and function after total cardiopulmonary bypass in neonatal piglets. American Journal of Respiratory and Critical Care Medicine, 159(2), 544-551. https://doi.org/10.1164/ajrccm.159.2.9803024