Vascular protection by angiotensin receptor antagonism involves differential vegf expression in both hemispheres after experimental stroke

Weihua Guan, Payaningal R. Somanath, Anna Koza, Anna Goc, Azza B. El-Remessy, Adviye Ergul, Maribeth H Johnson, Ahmed Alhusban, Sahar Soliman, Susan C. Fagan

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

We identified that the angiotensin receptor antagonist, candesartan, has profound neurovascular protective properties when administered after ischemic stroke and was associated with a proangiogenic state at least partly explained by vascular endothelial growth factor A (VEGFA). However, the spatial distribution of vascular endothelial growth factor (VEGF) isoforms and their receptors remained unknown. Protein analysis identified a significant increase in vascular endothelial grow factor B (VEGFB) in the cerebrospinal fluid (CSF) and the ischemic hemispheres (with increased VEGF receptor 1 activation) of treated animals (p<0.05) which was co-occurring with an increase in protein kinase B (Akt) phosphorylation (p<0.05). An increase in VEGFA protein in the contralesional hemisphere corresponded to a significant increase in vascular density at seven days (p<0.01) after stroke onset. Vascular restoration by candesartan after stroke maybe related to differential regional upregulation of VEGFB and VEGFA, promoting a "prosurvival state" in the ischemic hemisphere and angiogenesis in the contralesional side, respectively. These vascular changes in both hemispheres after effective treatment are likely to contribute to enhanced recovery after stroke.

Original languageEnglish (US)
Article numbere24551
JournalPLoS One
Volume6
Issue number9
DOIs
StatePublished - Sep 1 2011

Fingerprint

angiotensins
Angiotensin Receptors
stroke
blood vessels
Vascular Endothelial Growth Factor A
Blood Vessels
vascular endothelial growth factor A
Stroke
receptors
Enhanced recovery
Cerebrospinal fluid
Vascular Endothelial Growth Factor Receptor-1
Proto-Oncogene Proteins c-akt
vascular endothelial growth factor receptor-1
Phosphorylation
Angiotensin Receptor Antagonists
vascular endothelial growth factors
Spatial distribution
Restoration
cerebrospinal fluid

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Vascular protection by angiotensin receptor antagonism involves differential vegf expression in both hemispheres after experimental stroke. / Guan, Weihua; Somanath, Payaningal R.; Koza, Anna; Goc, Anna; El-Remessy, Azza B.; Ergul, Adviye; Johnson, Maribeth H; Alhusban, Ahmed; Soliman, Sahar; Fagan, Susan C.

In: PLoS One, Vol. 6, No. 9, e24551, 01.09.2011.

Research output: Contribution to journalArticle

Guan, W, Somanath, PR, Koza, A, Goc, A, El-Remessy, AB, Ergul, A, Johnson, MH, Alhusban, A, Soliman, S & Fagan, SC 2011, 'Vascular protection by angiotensin receptor antagonism involves differential vegf expression in both hemispheres after experimental stroke', PLoS One, vol. 6, no. 9, e24551. https://doi.org/10.1371/journal.pone.0024551
Guan, Weihua ; Somanath, Payaningal R. ; Koza, Anna ; Goc, Anna ; El-Remessy, Azza B. ; Ergul, Adviye ; Johnson, Maribeth H ; Alhusban, Ahmed ; Soliman, Sahar ; Fagan, Susan C. / Vascular protection by angiotensin receptor antagonism involves differential vegf expression in both hemispheres after experimental stroke. In: PLoS One. 2011 ; Vol. 6, No. 9.
@article{6926093b770146908b52d00891472c34,
title = "Vascular protection by angiotensin receptor antagonism involves differential vegf expression in both hemispheres after experimental stroke",
abstract = "We identified that the angiotensin receptor antagonist, candesartan, has profound neurovascular protective properties when administered after ischemic stroke and was associated with a proangiogenic state at least partly explained by vascular endothelial growth factor A (VEGFA). However, the spatial distribution of vascular endothelial growth factor (VEGF) isoforms and their receptors remained unknown. Protein analysis identified a significant increase in vascular endothelial grow factor B (VEGFB) in the cerebrospinal fluid (CSF) and the ischemic hemispheres (with increased VEGF receptor 1 activation) of treated animals (p<0.05) which was co-occurring with an increase in protein kinase B (Akt) phosphorylation (p<0.05). An increase in VEGFA protein in the contralesional hemisphere corresponded to a significant increase in vascular density at seven days (p<0.01) after stroke onset. Vascular restoration by candesartan after stroke maybe related to differential regional upregulation of VEGFB and VEGFA, promoting a {"}prosurvival state{"} in the ischemic hemisphere and angiogenesis in the contralesional side, respectively. These vascular changes in both hemispheres after effective treatment are likely to contribute to enhanced recovery after stroke.",
author = "Weihua Guan and Somanath, {Payaningal R.} and Anna Koza and Anna Goc and El-Remessy, {Azza B.} and Adviye Ergul and Johnson, {Maribeth H} and Ahmed Alhusban and Sahar Soliman and Fagan, {Susan C.}",
year = "2011",
month = "9",
day = "1",
doi = "10.1371/journal.pone.0024551",
language = "English (US)",
volume = "6",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "9",

}

TY - JOUR

T1 - Vascular protection by angiotensin receptor antagonism involves differential vegf expression in both hemispheres after experimental stroke

AU - Guan, Weihua

AU - Somanath, Payaningal R.

AU - Koza, Anna

AU - Goc, Anna

AU - El-Remessy, Azza B.

AU - Ergul, Adviye

AU - Johnson, Maribeth H

AU - Alhusban, Ahmed

AU - Soliman, Sahar

AU - Fagan, Susan C.

PY - 2011/9/1

Y1 - 2011/9/1

N2 - We identified that the angiotensin receptor antagonist, candesartan, has profound neurovascular protective properties when administered after ischemic stroke and was associated with a proangiogenic state at least partly explained by vascular endothelial growth factor A (VEGFA). However, the spatial distribution of vascular endothelial growth factor (VEGF) isoforms and their receptors remained unknown. Protein analysis identified a significant increase in vascular endothelial grow factor B (VEGFB) in the cerebrospinal fluid (CSF) and the ischemic hemispheres (with increased VEGF receptor 1 activation) of treated animals (p<0.05) which was co-occurring with an increase in protein kinase B (Akt) phosphorylation (p<0.05). An increase in VEGFA protein in the contralesional hemisphere corresponded to a significant increase in vascular density at seven days (p<0.01) after stroke onset. Vascular restoration by candesartan after stroke maybe related to differential regional upregulation of VEGFB and VEGFA, promoting a "prosurvival state" in the ischemic hemisphere and angiogenesis in the contralesional side, respectively. These vascular changes in both hemispheres after effective treatment are likely to contribute to enhanced recovery after stroke.

AB - We identified that the angiotensin receptor antagonist, candesartan, has profound neurovascular protective properties when administered after ischemic stroke and was associated with a proangiogenic state at least partly explained by vascular endothelial growth factor A (VEGFA). However, the spatial distribution of vascular endothelial growth factor (VEGF) isoforms and their receptors remained unknown. Protein analysis identified a significant increase in vascular endothelial grow factor B (VEGFB) in the cerebrospinal fluid (CSF) and the ischemic hemispheres (with increased VEGF receptor 1 activation) of treated animals (p<0.05) which was co-occurring with an increase in protein kinase B (Akt) phosphorylation (p<0.05). An increase in VEGFA protein in the contralesional hemisphere corresponded to a significant increase in vascular density at seven days (p<0.01) after stroke onset. Vascular restoration by candesartan after stroke maybe related to differential regional upregulation of VEGFB and VEGFA, promoting a "prosurvival state" in the ischemic hemisphere and angiogenesis in the contralesional side, respectively. These vascular changes in both hemispheres after effective treatment are likely to contribute to enhanced recovery after stroke.

UR - http://www.scopus.com/inward/record.url?scp=80052413036&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80052413036&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0024551

DO - 10.1371/journal.pone.0024551

M3 - Article

C2 - 21912702

AN - SCOPUS:80052413036

VL - 6

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 9

M1 - e24551

ER -