Vascular responses to serotonin in steroid hypertensive rats

Thomas E. Mecca, R Clinton Webb

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

This study investigates the mechanism responsible for increased vascular sensitivity to serotonin in deoxycorticosterone acetate (DOCA)-salt hypertension. Femoral arteries from normotensive and hypertensive rats were excised and cut into helical strips for isometric force recording. Dose-response curves to serotonin were shifted significantly to the left in arteries from DOCA-salt hypertensive rats compared to those from normotensive rats (ED^: DOCA = 7.1 x 10~8M; control = 27 x 10~8M). The partial agonistic properties of methysergide were increased in femoral arteries from DOCA-salt hypertensive rats. The competitive antagonism of serotonin by methysergide or ketanserin was similar in arteries from control and DOCA-salt hypertensive rats (pA2: methysergide, control = 10.4, DOCA = 10.5; and ketanserin, control = 10.4, DOCA = 10.4). After cellular calcium (Ca) depletion with EGTA, dose-response curves to Ca were obtained in the presence of serotonin (5.7 x 10~5M). The Ca sensitivity of vessels from hypertensive rats was not statistically different from that in arteries from normotensive rats. Contractile responses to serotonin in calciumfree solution following loading of a cellular store with Ca were 50% greater in arteries from DOCA hypertensive rats. These results suggest that the enhanced sensitivity to serotonin in DOCA-salt hypertensive rats is not related to a change in receptor affinity nor to an alteration in transmembrane movement of Ca following receptor activation. The increased serotonin sensitivity is related to an altered mobilization of Ca from a cellular store.

Original languageEnglish (US)
Pages (from-to)887-892
Number of pages6
JournalHypertension
Volume6
Issue number6
DOIs
StatePublished - Jan 1 1984
Externally publishedYes

Fingerprint

Desoxycorticosterone
Blood Vessels
Serotonin
Acetates
Steroids
Methysergide
Salts
Calcium
Arteries
Ketanserin
Femoral Artery
Calcium-Sensing Receptors
Egtazic Acid
Hypertension

Keywords

  • Calcium
  • Isolated arterial strips
  • Ketanserin
  • Methysergide
  • Vascular sensitivity

ASJC Scopus subject areas

  • Internal Medicine

Cite this

Vascular responses to serotonin in steroid hypertensive rats. / Mecca, Thomas E.; Webb, R Clinton.

In: Hypertension, Vol. 6, No. 6, 01.01.1984, p. 887-892.

Research output: Contribution to journalArticle

Mecca, Thomas E. ; Webb, R Clinton. / Vascular responses to serotonin in steroid hypertensive rats. In: Hypertension. 1984 ; Vol. 6, No. 6. pp. 887-892.
@article{3a46ca8853ae4c1f9a4a0546c605bfec,
title = "Vascular responses to serotonin in steroid hypertensive rats",
abstract = "This study investigates the mechanism responsible for increased vascular sensitivity to serotonin in deoxycorticosterone acetate (DOCA)-salt hypertension. Femoral arteries from normotensive and hypertensive rats were excised and cut into helical strips for isometric force recording. Dose-response curves to serotonin were shifted significantly to the left in arteries from DOCA-salt hypertensive rats compared to those from normotensive rats (ED^: DOCA = 7.1 x 10~8M; control = 27 x 10~8M). The partial agonistic properties of methysergide were increased in femoral arteries from DOCA-salt hypertensive rats. The competitive antagonism of serotonin by methysergide or ketanserin was similar in arteries from control and DOCA-salt hypertensive rats (pA2: methysergide, control = 10.4, DOCA = 10.5; and ketanserin, control = 10.4, DOCA = 10.4). After cellular calcium (Ca) depletion with EGTA, dose-response curves to Ca were obtained in the presence of serotonin (5.7 x 10~5M). The Ca sensitivity of vessels from hypertensive rats was not statistically different from that in arteries from normotensive rats. Contractile responses to serotonin in calciumfree solution following loading of a cellular store with Ca were 50{\%} greater in arteries from DOCA hypertensive rats. These results suggest that the enhanced sensitivity to serotonin in DOCA-salt hypertensive rats is not related to a change in receptor affinity nor to an alteration in transmembrane movement of Ca following receptor activation. The increased serotonin sensitivity is related to an altered mobilization of Ca from a cellular store.",
keywords = "Calcium, Isolated arterial strips, Ketanserin, Methysergide, Vascular sensitivity",
author = "Mecca, {Thomas E.} and Webb, {R Clinton}",
year = "1984",
month = "1",
day = "1",
doi = "10.1161/01.HYP.6.6.887",
language = "English (US)",
volume = "6",
pages = "887--892",
journal = "Hypertension",
issn = "0194-911X",
publisher = "Lippincott Williams and Wilkins",
number = "6",

}

TY - JOUR

T1 - Vascular responses to serotonin in steroid hypertensive rats

AU - Mecca, Thomas E.

AU - Webb, R Clinton

PY - 1984/1/1

Y1 - 1984/1/1

N2 - This study investigates the mechanism responsible for increased vascular sensitivity to serotonin in deoxycorticosterone acetate (DOCA)-salt hypertension. Femoral arteries from normotensive and hypertensive rats were excised and cut into helical strips for isometric force recording. Dose-response curves to serotonin were shifted significantly to the left in arteries from DOCA-salt hypertensive rats compared to those from normotensive rats (ED^: DOCA = 7.1 x 10~8M; control = 27 x 10~8M). The partial agonistic properties of methysergide were increased in femoral arteries from DOCA-salt hypertensive rats. The competitive antagonism of serotonin by methysergide or ketanserin was similar in arteries from control and DOCA-salt hypertensive rats (pA2: methysergide, control = 10.4, DOCA = 10.5; and ketanserin, control = 10.4, DOCA = 10.4). After cellular calcium (Ca) depletion with EGTA, dose-response curves to Ca were obtained in the presence of serotonin (5.7 x 10~5M). The Ca sensitivity of vessels from hypertensive rats was not statistically different from that in arteries from normotensive rats. Contractile responses to serotonin in calciumfree solution following loading of a cellular store with Ca were 50% greater in arteries from DOCA hypertensive rats. These results suggest that the enhanced sensitivity to serotonin in DOCA-salt hypertensive rats is not related to a change in receptor affinity nor to an alteration in transmembrane movement of Ca following receptor activation. The increased serotonin sensitivity is related to an altered mobilization of Ca from a cellular store.

AB - This study investigates the mechanism responsible for increased vascular sensitivity to serotonin in deoxycorticosterone acetate (DOCA)-salt hypertension. Femoral arteries from normotensive and hypertensive rats were excised and cut into helical strips for isometric force recording. Dose-response curves to serotonin were shifted significantly to the left in arteries from DOCA-salt hypertensive rats compared to those from normotensive rats (ED^: DOCA = 7.1 x 10~8M; control = 27 x 10~8M). The partial agonistic properties of methysergide were increased in femoral arteries from DOCA-salt hypertensive rats. The competitive antagonism of serotonin by methysergide or ketanserin was similar in arteries from control and DOCA-salt hypertensive rats (pA2: methysergide, control = 10.4, DOCA = 10.5; and ketanserin, control = 10.4, DOCA = 10.4). After cellular calcium (Ca) depletion with EGTA, dose-response curves to Ca were obtained in the presence of serotonin (5.7 x 10~5M). The Ca sensitivity of vessels from hypertensive rats was not statistically different from that in arteries from normotensive rats. Contractile responses to serotonin in calciumfree solution following loading of a cellular store with Ca were 50% greater in arteries from DOCA hypertensive rats. These results suggest that the enhanced sensitivity to serotonin in DOCA-salt hypertensive rats is not related to a change in receptor affinity nor to an alteration in transmembrane movement of Ca following receptor activation. The increased serotonin sensitivity is related to an altered mobilization of Ca from a cellular store.

KW - Calcium

KW - Isolated arterial strips

KW - Ketanserin

KW - Methysergide

KW - Vascular sensitivity

UR - http://www.scopus.com/inward/record.url?scp=0021710391&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021710391&partnerID=8YFLogxK

U2 - 10.1161/01.HYP.6.6.887

DO - 10.1161/01.HYP.6.6.887

M3 - Article

VL - 6

SP - 887

EP - 892

JO - Hypertension

JF - Hypertension

SN - 0194-911X

IS - 6

ER -