Vascular Responses to Sodium Arachidonate in Experimental Hypertension

Warren E. Lockette, R Clinton Webb

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

This study characterizes vascular responsiveness to sodium arachidonate (C 20:4) in four models of hypertension [deoxycorticosterone acetate (DOCA) hypertensive rats, two kidney-one clip (2K-1C) renal hypertensive rats, spontaneously hypertensive rats (SHR), and psychosocial hypertensive mice]. Isolated arterial strips (aorta, mesenteric artery, tail artery) were equilibrated under optimal resting tension in physiological salt solution for measurement of isometric force generation. Dose-response curves to arachidonate (10−10 to 10−4 g/ml) in arteries from DOCA and 2K-1C hypertensive rats were shifted to the left compared to those in arteries from control rats. In arteries from SHR and psychosocial hypertensive mice, the dose-response relationships were unchanged compared to normotensive values. Arteries from DOCA hypertensive and 2K-1C hypertensive rats developed greater maximal contractile responses to arachidonate than controls; maximal responses in arteries from SHR and psychosocial hypertensive mice were unchanged compared to normotensive values. Contractions to arachidonate were inhibited by indomethacin (0.5 and 5 μg/ml) and by aspirin (5 and 50 μg/ml). The fatty acid, oleate (C 18:1), had no effect on the contractile state of the arteries, whereas prostaglandin F2α caused contraction. These results indicate altered responsiveness to exogenous arachidonate in arteries from DOCA and 2K-1C hypertensive rats, but not in arteries from SHR and psychosocial hypertensive mice.

Original languageEnglish (US)
Pages (from-to)536-545
Number of pages10
JournalProceedings of the Society for Experimental Biology and Medicine
Volume178
Issue number4
DOIs
StatePublished - Jan 1 1985

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Arachidonic Acid
Blood Vessels
Rats
Arteries
Hypertension
Desoxycorticosterone
Inbred SHR Rats
Acetates
Rat control
Kidney
Mesenteric Arteries
Dinoprost
Oleic Acid
Surgical Instruments
Indomethacin
Aspirin
Aorta
Tail
Fatty Acids
Salts

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Vascular Responses to Sodium Arachidonate in Experimental Hypertension. / Lockette, Warren E.; Webb, R Clinton.

In: Proceedings of the Society for Experimental Biology and Medicine, Vol. 178, No. 4, 01.01.1985, p. 536-545.

Research output: Contribution to journalArticle

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