Vasopressin-stimulated electrogenic sodium transport in a6 cells is linked to a Ca2+-mobilizing signal mechanism

John P. Hayslett, Lawrence J. Macala, Joan I. Smallwood, Leena Kalghatgi, Jose Gassala-Herraiz, Carlos M Isales

Research output: Contribution to journalArticle

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Abstract

Vasopressin is known to activate two types of cell surface receptors; V2, coupled to adenylate cyclase, and V1, linked to a Ca2+-dependent transduction system. We investigated whether arginine vasopressin (AVP) stimulation of electrogenic sodium transport in A6 cells, derived from Xenopus laevis, is mediated by activation of either one or both types of AVP-specific receptors. AVP caused a rapid increase in electrogenic sodium transport, reflected by the transepithelial potential difference (VT) and equivalent short circuit current (Ieq) measurements. AVP also rapidly increased intracellular Ca2+ (Ca2+i) and total inositol trisphosphate. The increase in Ieq was dependent on the rise in (Ca2+i), because 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid (BAPTA) dose-dependently inhibited the Ieq response. There was no evidence, however, that activation of adenylate cyclase mediated AVP-stimulated Ieq; transport was not inhibited after AVP-induced activation of adenylate cyclase was abolished by 2′,5′-dideoxyadenosine or when cAMP-dependent protein kinase (PKA) activity was abolished by the specific PKA inhibitor IP20. Further studies showed that although both forskolin and 8-(4-chlorophenylthio)-cAMP stimulated Ieq, this occurred by mechanisms independent of PKA activation. These results indicate that AVP-stimulated Na+ transport is mediated by a V1 receptor and a Ca2+- dependent mechanism.

Original languageEnglish (US)
Pages (from-to)16082-16088
Number of pages7
JournalJournal of Biological Chemistry
Volume270
Issue number27
DOIs
StatePublished - Jul 7 1995

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Arginine Vasopressin
Vasopressins
Sodium
Adenylyl Cyclases
Chemical activation
Vasopressin Receptors
Dideoxyadenosine
Ethane
Xenopus laevis
Cell Surface Receptors
Colforsin
Inositol
Protein Kinase Inhibitors
Cyclic AMP-Dependent Protein Kinases
Protein Kinases
Electric current measurement
Short circuit currents
Acids

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Vasopressin-stimulated electrogenic sodium transport in a6 cells is linked to a Ca2+-mobilizing signal mechanism. / Hayslett, John P.; Macala, Lawrence J.; Smallwood, Joan I.; Kalghatgi, Leena; Gassala-Herraiz, Jose; Isales, Carlos M.

In: Journal of Biological Chemistry, Vol. 270, No. 27, 07.07.1995, p. 16082-16088.

Research output: Contribution to journalArticle

Hayslett, John P. ; Macala, Lawrence J. ; Smallwood, Joan I. ; Kalghatgi, Leena ; Gassala-Herraiz, Jose ; Isales, Carlos M. / Vasopressin-stimulated electrogenic sodium transport in a6 cells is linked to a Ca2+-mobilizing signal mechanism. In: Journal of Biological Chemistry. 1995 ; Vol. 270, No. 27. pp. 16082-16088.
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