VCAM-1 expression in adult hematopoietic and nonhematopoietic cells is controlled by tissue-inductive signals and reflects their developmental origin

Tatiana Ulyanova, Linda M. Scott, Gregory V. Priestley, Yi Jiang, Betty Nakamoto, Pandelakis Koni, Thalia Papayannopoulou

Research output: Contribution to journalArticle

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Abstract

Although expression of vascular cell adhesion molecule 1 (VCAM-1) in endothelial cells and its functional implications have been previously appreciated, VCAM-1 expression in other than endothelial cells, especially hematopoietic cells, has been recently recognized and has not been explored in detail. Using normal mice and mice with a conditional ablation of VCAM-1 through a Tie2-driven cretransgene, we have studied the biodistribution and the pattern of VCAM-1 expression in circulating versus tissue-residing cells before and after their enforced mobilization. In the normal mouse, both at basal hematopoiesis or following mobilization, VCAM-1 expression is confined to myeloid cells residing in hematopoietic tissues, whereas free cells in circulation or in body cavities are devoid of VCAM-1 messenger RNA (mRNA) and protein. However, following culture, proliferating myeloid cells, but not lymphoid cells, express VCAM-1. In the VCAM-1-ablated mouse, there is an increase in circulating progenitors as a consequence of their ongoing release from bone marrow, a process enhanced by splenectomy. We postulate that the main mechanism leading to their release is the ablation of VCAM-1 by fibroblastic and by endothelial cells. Ablation of VCAM-1 in fibroblasts by Tie2-driven ere is a novel finding and likely denotes their developmental ancestry by Tie2-expressing (mesenchymal?) progenitor cells during development.

Original languageEnglish (US)
Pages (from-to)86-94
Number of pages9
JournalBlood
Volume106
Issue number1
DOIs
StatePublished - Jul 1 2005

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Vascular Cell Adhesion Molecule-1
Tissue
Endothelial cells
Ablation
Endothelial Cells
Myeloid Cells
Hematopoiesis
Splenectomy
Fibroblasts
Mesenchymal Stromal Cells
Bone
Bone Marrow
Lymphocytes
Messenger RNA

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

VCAM-1 expression in adult hematopoietic and nonhematopoietic cells is controlled by tissue-inductive signals and reflects their developmental origin. / Ulyanova, Tatiana; Scott, Linda M.; Priestley, Gregory V.; Jiang, Yi; Nakamoto, Betty; Koni, Pandelakis; Papayannopoulou, Thalia.

In: Blood, Vol. 106, No. 1, 01.07.2005, p. 86-94.

Research output: Contribution to journalArticle

Ulyanova, T, Scott, LM, Priestley, GV, Jiang, Y, Nakamoto, B, Koni, P & Papayannopoulou, T 2005, 'VCAM-1 expression in adult hematopoietic and nonhematopoietic cells is controlled by tissue-inductive signals and reflects their developmental origin', Blood, vol. 106, no. 1, pp. 86-94. https://doi.org/10.1182/blood-2004-09-3417
Ulyanova, Tatiana ; Scott, Linda M. ; Priestley, Gregory V. ; Jiang, Yi ; Nakamoto, Betty ; Koni, Pandelakis ; Papayannopoulou, Thalia. / VCAM-1 expression in adult hematopoietic and nonhematopoietic cells is controlled by tissue-inductive signals and reflects their developmental origin. In: Blood. 2005 ; Vol. 106, No. 1. pp. 86-94.
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