VEGF differentially activates STAT3 in microvascular endothelial cells.

Manuela Bartoli, Dan Platt, Tahira Lemtalsi, Xiaolin Gu, Steven E. Brooks, Mario B Marrero, Ruth B Caldwell

Research output: Contribution to journalArticle

129 Citations (Scopus)

Abstract

Increased VEGF expression is found in several pathologies characterized by abnormal angiogenesis. Previous studies have shown that the transcription factor STAT3 mediates VEGF gene transcription and its activation. In this study, Western analysis and confocal immunocytochemistry were used to examine STAT3 activation in retinal microvascular endothelial cells (BREC). We found that VEGF rapidly induces STAT3 tyrosine phosphorylation and nuclear translocation. Immunoprecipitation studies also showed that VEGF forms a complex with VEGFR2 only in BREC and not in aortic macrovascular endothelial cells (BAEC). In addition, quantitative real-time RT-PCR analysis of VEGF-induced VEGF expression showed a significant increase in specific mRNA formation only in BREC and not in BAEC, and this effect was significantly reduced by antisense-mediated reduction of STAT3 expression. Furthermore, studies conducted in human dermal microvascular endothelial cells (HDMEC) showed that, in this endothelial cell type, VEGF autocrine expression is also accompanied by STAT3 activation as in BREC. In this study we showed that VEGF can differentially induce STAT3 activation in micro- versus macro-vascular endothelial cells and that this effect is linked to VEGFR2/STAT3 complex formation, which correlates with VEGF autocrine ability to stimulate its own gene expression.

Original languageEnglish (US)
Pages (from-to)1562-1564
Number of pages3
JournalThe FASEB journal : official publication of the Federation of American Societies for Experimental Biology
Volume17
Issue number11
StatePublished - Jan 1 2003

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Endothelial cells
Vascular Endothelial Growth Factor A
Endothelial Cells
Chemical activation
STAT3 Transcription Factor
Phosphorylation
Pathology
Transcription
Immunoprecipitation
Gene expression
Transcriptional Activation
Macros
Tyrosine
Real-Time Polymerase Chain Reaction
Genes
Immunohistochemistry
Gene Expression
Messenger RNA
Skin

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

VEGF differentially activates STAT3 in microvascular endothelial cells. / Bartoli, Manuela; Platt, Dan; Lemtalsi, Tahira; Gu, Xiaolin; Brooks, Steven E.; Marrero, Mario B; Caldwell, Ruth B.

In: The FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 17, No. 11, 01.01.2003, p. 1562-1564.

Research output: Contribution to journalArticle

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AU - Marrero, Mario B

AU - Caldwell, Ruth B

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