VEGF expression by mesenchymal stem cells contributes to angiogenesis in pancreatic carcinoma

B. M. Beckermann, Georgios Kallifatidis, A. Groth, D. Frommhold, A. Apel, J. Mattern, A. V. Salnikov, G. Moldenhauer, W. Wagner, A. Diehlmann, R. Saffrich, M. Schubert, A. D. Ho, N. Giese, M. W. Büchler, H. Friess, P. Büchler, I. Herr

Research output: Contribution to journalArticle

242 Citations (Scopus)

Abstract

Little is known about the factors that enable the mobilisation of human mesenchymal stem cells (MSC) from the bone marrow into the blood stream and their recruitment to and retention in the tumour. We found specific migration of MSC towards growth factors present in pancreatic tumours, such as PDGF, EGF, VEGF and specific inhibitors Glivec, Erbitux and Avastin interfered with migration. Within a few hours, MSC migrated into spheroids consisting of pancreatic cancer cells, fibroblasts and endothelial cells as measured by time-lapse microscopy. Supernatant from subconfluent MSC increased sprouting of HUVEC due to VEGF production by MSC itself as demonstrated by RT-PCR and ELISA. Only few MSCs were differentiated into endothelial cells in vitro, whereas in vivo differentiation was not observed. Lentiviral GFP-marked MSCs, injected in nude mice xenografted with orthotopic pancreatic tumours, preferentially migrated into the tumours as observed by FACS analysis of green fluorescent cells. By immunofluorescence and intravital microscopic studies, we found the interaction of MSC with the endothelium of blood vessels. Mesenchymal stem cells supported tumour angiogenesis in vivo, that is CD31+ vessel density was increased after the transfer of MSC compared with siVEGF-MSC. Our data demonstrate the migration of MSC toward tumour vessels and suggest a supportive role in angiogenesis.

Original languageEnglish (US)
Pages (from-to)622-631
Number of pages10
JournalBritish Journal of Cancer
Volume99
Issue number4
DOIs
StatePublished - Aug 19 2008

Fingerprint

Mesenchymal Stromal Cells
Vascular Endothelial Growth Factor A
Neoplasms
Endothelial Cells
Pancreatic Carcinoma
Pancreatic Neoplasms
Epidermal Growth Factor
Nude Mice
Endothelium
Fluorescent Antibody Technique
Blood Vessels
Microscopy
Intercellular Signaling Peptides and Proteins
Fibroblasts
Bone Marrow
Enzyme-Linked Immunosorbent Assay
Polymerase Chain Reaction

Keywords

  • Angiogenesis
  • Lentivirus
  • MSC
  • Pancreas
  • VEGF

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

VEGF expression by mesenchymal stem cells contributes to angiogenesis in pancreatic carcinoma. / Beckermann, B. M.; Kallifatidis, Georgios; Groth, A.; Frommhold, D.; Apel, A.; Mattern, J.; Salnikov, A. V.; Moldenhauer, G.; Wagner, W.; Diehlmann, A.; Saffrich, R.; Schubert, M.; Ho, A. D.; Giese, N.; Büchler, M. W.; Friess, H.; Büchler, P.; Herr, I.

In: British Journal of Cancer, Vol. 99, No. 4, 19.08.2008, p. 622-631.

Research output: Contribution to journalArticle

Beckermann, BM, Kallifatidis, G, Groth, A, Frommhold, D, Apel, A, Mattern, J, Salnikov, AV, Moldenhauer, G, Wagner, W, Diehlmann, A, Saffrich, R, Schubert, M, Ho, AD, Giese, N, Büchler, MW, Friess, H, Büchler, P & Herr, I 2008, 'VEGF expression by mesenchymal stem cells contributes to angiogenesis in pancreatic carcinoma', British Journal of Cancer, vol. 99, no. 4, pp. 622-631. https://doi.org/10.1038/sj.bjc.6604508
Beckermann, B. M. ; Kallifatidis, Georgios ; Groth, A. ; Frommhold, D. ; Apel, A. ; Mattern, J. ; Salnikov, A. V. ; Moldenhauer, G. ; Wagner, W. ; Diehlmann, A. ; Saffrich, R. ; Schubert, M. ; Ho, A. D. ; Giese, N. ; Büchler, M. W. ; Friess, H. ; Büchler, P. ; Herr, I. / VEGF expression by mesenchymal stem cells contributes to angiogenesis in pancreatic carcinoma. In: British Journal of Cancer. 2008 ; Vol. 99, No. 4. pp. 622-631.
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