VEGF signaling through NADPH oxidase-derived ROS

Research output: Contribution to journalArticle

174 Citations (Scopus)

Abstract

Angiogenesis is a key process involved in normal development and wound repair, as well as ischemic heart and limb diseases, and atherosclerosis. Vascular endothelial growth factor (VEGF), a potent angiogenesis factor, stimulates proliferation, migration, and tube formation of endothelial cells (ECs), primarily through the VEGF receptor type2 (VEGFR2). Reactive oxygen species (ROS) function as signaling molecules to mediate biological responses. In ECs, NADPH oxidase is one of the major sources of ROS and consists of catalytic sub-units (Nox1, Nox2, and Nox4), p22phox, p47phox, p67phox, and the small GTPase Rac1. VEGF stimulates ROS production via activation of gp91phox (Nox2)-based NADPH oxidase, and ROS are involved in VEGFR2-mediated signaling linked to EC migration and proliferation. Moreover, ROS derived from NADPH oxidase are involved in postnatal angiogenesis. Localizing NADPH oxidase and its regulators at the specific subcellular compartment is an important mechanism for activating specific redox signaling events. This review focuses on a role of NADPH oxidase-derived ROS in angiogenesis and critical regulators involved in generation of spatially and temporally restricted ROS-dependent VEGF signaling at leading edge, focal adhesions/complexes, caveolae/lipid rafts, and cell-cell junctions in ECs. Understanding these mechanisms should facilitate the development of new therapeutic strategies to modulate new blood vessel formation.

Original languageEnglish (US)
Pages (from-to)731-739
Number of pages9
JournalAntioxidants and Redox Signaling
Volume9
Issue number6
DOIs
StatePublished - Jun 1 2007
Externally publishedYes

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NADPH Oxidase
Vascular Endothelial Growth Factor A
Reactive Oxygen Species
Endothelial cells
Endothelial Cells
Vascular Endothelial Growth Factor Receptor
Caveolae
Focal Adhesions
Intercellular Junctions
Monomeric GTP-Binding Proteins
Angiogenesis Inducing Agents
Blood vessels
Oxidation-Reduction
Cell Movement
Myocardial Ischemia
Blood Vessels
Atherosclerosis
Repair
Adhesion
Extremities

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

Cite this

VEGF signaling through NADPH oxidase-derived ROS. / Fukai, Masuko.

In: Antioxidants and Redox Signaling, Vol. 9, No. 6, 01.06.2007, p. 731-739.

Research output: Contribution to journalArticle

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