Abstract
Objective and design: The present study was aimed to evaluate the anti-inflammatory potentials of Vespa tropica (VT) venom and its isolated peptides. Effects of whole venom and its two peptides (Vt1512 and Vt1386) on lipopolysaccharide (LPS) challenged BV-2 murine microglial cells was evaluated. Materials: Mouse microglial cell line, BV-2 and crude venom extract as well as purified peptides from VT along with LPS from Salmonella enterica were used for the studies. Treatment: BV-2 cells were treated with 500 ng/ml of LPS and different doses of crude wasp venom as well as purified peptides. Methods: We used immunoblotting, cytokine bead arrays and fluorescence activated cell sorter (FACS) to evaluate the levels of various proteins, cytokines and reactive oxygen species (ROS). Results: Our studies suggest that treatment with whole venom significantly reduces oxidative stress and LPS-stimulated activation of microglia. Also, purified peptides from crude venom exhibited potential anti-inflammatory properties. Further, whole venom was found to be targeting Akt and p38 MAPK pathways, leading to suppressed NF-κB phosphorylation in LPS challenged BV-2 cells. Conclusions: VT venom possesses anti-inflammatory properties and can be further explored for their therapeutic potential in treating various inflammatory conditions of the central nervous system (CNS).
| Original language | English (US) |
|---|---|
| Pages (from-to) | 657-665 |
| Number of pages | 9 |
| Journal | Inflammation Research |
| Volume | 63 |
| Issue number | 8 |
| DOIs | |
| State | Published - Jan 1 2014 |
| Externally published | Yes |
Fingerprint
Keywords
- Cytokines
- Lipopolysaccharide
- Microglia
- Neuroinflammation
- Wasp venom
ASJC Scopus subject areas
- Immunology
- Pharmacology
Cite this
Vespa tropica venom suppresses lipopolysaccharide-mediated secretion of pro-inflammatory cyto-chemokines by abrogating nuclear factor-κ B activation in microglia. / Kaushik, Deepak Kumar; Thounaojam, Menaka; Mitra, Arinjay; Basu, Anirban.
In: Inflammation Research, Vol. 63, No. 8, 01.01.2014, p. 657-665.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Vespa tropica venom suppresses lipopolysaccharide-mediated secretion of pro-inflammatory cyto-chemokines by abrogating nuclear factor-κ B activation in microglia
AU - Kaushik, Deepak Kumar
AU - Thounaojam, Menaka
AU - Mitra, Arinjay
AU - Basu, Anirban
PY - 2014/1/1
Y1 - 2014/1/1
N2 - Objective and design: The present study was aimed to evaluate the anti-inflammatory potentials of Vespa tropica (VT) venom and its isolated peptides. Effects of whole venom and its two peptides (Vt1512 and Vt1386) on lipopolysaccharide (LPS) challenged BV-2 murine microglial cells was evaluated. Materials: Mouse microglial cell line, BV-2 and crude venom extract as well as purified peptides from VT along with LPS from Salmonella enterica were used for the studies. Treatment: BV-2 cells were treated with 500 ng/ml of LPS and different doses of crude wasp venom as well as purified peptides. Methods: We used immunoblotting, cytokine bead arrays and fluorescence activated cell sorter (FACS) to evaluate the levels of various proteins, cytokines and reactive oxygen species (ROS). Results: Our studies suggest that treatment with whole venom significantly reduces oxidative stress and LPS-stimulated activation of microglia. Also, purified peptides from crude venom exhibited potential anti-inflammatory properties. Further, whole venom was found to be targeting Akt and p38 MAPK pathways, leading to suppressed NF-κB phosphorylation in LPS challenged BV-2 cells. Conclusions: VT venom possesses anti-inflammatory properties and can be further explored for their therapeutic potential in treating various inflammatory conditions of the central nervous system (CNS).
AB - Objective and design: The present study was aimed to evaluate the anti-inflammatory potentials of Vespa tropica (VT) venom and its isolated peptides. Effects of whole venom and its two peptides (Vt1512 and Vt1386) on lipopolysaccharide (LPS) challenged BV-2 murine microglial cells was evaluated. Materials: Mouse microglial cell line, BV-2 and crude venom extract as well as purified peptides from VT along with LPS from Salmonella enterica were used for the studies. Treatment: BV-2 cells were treated with 500 ng/ml of LPS and different doses of crude wasp venom as well as purified peptides. Methods: We used immunoblotting, cytokine bead arrays and fluorescence activated cell sorter (FACS) to evaluate the levels of various proteins, cytokines and reactive oxygen species (ROS). Results: Our studies suggest that treatment with whole venom significantly reduces oxidative stress and LPS-stimulated activation of microglia. Also, purified peptides from crude venom exhibited potential anti-inflammatory properties. Further, whole venom was found to be targeting Akt and p38 MAPK pathways, leading to suppressed NF-κB phosphorylation in LPS challenged BV-2 cells. Conclusions: VT venom possesses anti-inflammatory properties and can be further explored for their therapeutic potential in treating various inflammatory conditions of the central nervous system (CNS).
KW - Cytokines
KW - Lipopolysaccharide
KW - Microglia
KW - Neuroinflammation
KW - Wasp venom
UR - http://www.scopus.com/inward/record.url?scp=84904321436&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84904321436&partnerID=8YFLogxK
U2 - 10.1007/s00011-014-0738-0
DO - 10.1007/s00011-014-0738-0
M3 - Article
VL - 63
SP - 657
EP - 665
JO - Inflammation Research
JF - Inflammation Research
SN - 1023-3830
IS - 8
ER -