TY - JOUR
T1 - Virucidal activities of novel hand hygiene and surface disinfectant formulations containing EGCG-palmitates (EC16)
AU - Dickinson, Douglas
AU - Marsh, Bianca
AU - Shao, Xueling
AU - Liu, Emma
AU - Sampath, Lester
AU - Yao, Bo
AU - Jiang, Xiaocui
AU - Hsu, Stephen
N1 - Funding Information:
Conflicts of interest: Salary supported by grants funded by National Institute of Allergy and Infectious Diseases: Douglas Dickinson, Bianca Marsh, Xueling Shao and Stephen Hsu. No conflict of interest: Emma Liu, Lester Sampath, Bo Yao, Xiaocui Jiang.
Funding Information:
Funding: This study was supported by grants from the National Institute of Allergy and Infectious Diseases (NIAID) to Camellix, LLC ( 1R41AI124738-01A1 , 2R42AI124738-02 , and 5R42AI124738-03 ), Augusta University Office of Innovation Commercialization, and Augusta University Research Institute . Research reported in this publication was supported by the National Institute of Allergy and Infectious Diseases of the National Institutes of Health under Award Number R42AI124738 . The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institute of Health.
Publisher Copyright:
© 2022 Association for Professionals in Infection Control and Epidemiology, Inc.
PY - 2022/11
Y1 - 2022/11
N2 - Background: Non-toxic hand hygiene and surface disinfectant products with virucidal activity against alcohol-resistant nonenveloped norovirus are in urgent need. Method: Alcohol-based formulations were made with epigallocatechin-3-gallate-palmitate (EC16), an FDA accepted food additive. Based on in-house testing of formulations, 3 prototypes, PTV80 hand gel, PST70 surface disinfectant spray and PST70 surface disinfectant wipe, were selected from in-house tests for independent testing at GLP (good laboratory practice) laboratories according to EN 14476:2019 (hand gel), ASTM test method E1053-20 (spray), and ASTM E2362-15, E1053, and ASTM E2896-12 (wipe). Results: The PTV80 hand gel prototype demonstrated a >99.999% reduction of murine norovirus S99 infectivity in 60 seconds. Carrier testing of the PST70 surface spray and surface wipe demonstrated reduction of feline calicivirus infectivity by >99.99% in 60 seconds. In addition, testing with human coronavirus and human herpes simplex virus demonstrated >99.99% efficacy in 60 seconds, consistent with broad spectrum virucidal activity. Conclusions: The novel non-toxic prototypes containing EC16 were found to be suitable for use in future hand sanitizer gel, surface disinfectant spray and wipe products against norovirus. Products based on these formulations could be used safely to help prevent and control norovirus and other emerging virus outbreaks, pending future studies.
AB - Background: Non-toxic hand hygiene and surface disinfectant products with virucidal activity against alcohol-resistant nonenveloped norovirus are in urgent need. Method: Alcohol-based formulations were made with epigallocatechin-3-gallate-palmitate (EC16), an FDA accepted food additive. Based on in-house testing of formulations, 3 prototypes, PTV80 hand gel, PST70 surface disinfectant spray and PST70 surface disinfectant wipe, were selected from in-house tests for independent testing at GLP (good laboratory practice) laboratories according to EN 14476:2019 (hand gel), ASTM test method E1053-20 (spray), and ASTM E2362-15, E1053, and ASTM E2896-12 (wipe). Results: The PTV80 hand gel prototype demonstrated a >99.999% reduction of murine norovirus S99 infectivity in 60 seconds. Carrier testing of the PST70 surface spray and surface wipe demonstrated reduction of feline calicivirus infectivity by >99.99% in 60 seconds. In addition, testing with human coronavirus and human herpes simplex virus demonstrated >99.99% efficacy in 60 seconds, consistent with broad spectrum virucidal activity. Conclusions: The novel non-toxic prototypes containing EC16 were found to be suitable for use in future hand sanitizer gel, surface disinfectant spray and wipe products against norovirus. Products based on these formulations could be used safely to help prevent and control norovirus and other emerging virus outbreaks, pending future studies.
KW - Alcohol-resistant microorganisms
KW - Green tea polyphenols
KW - Non-toxic formulations
KW - Norovirus
KW - Novel antiseptics
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U2 - 10.1016/j.ajic.2022.05.027
DO - 10.1016/j.ajic.2022.05.027
M3 - Article
C2 - 35671844
AN - SCOPUS:85134764366
VL - 50
SP - 1212
EP - 1219
JO - American Journal of Infection Control
JF - American Journal of Infection Control
SN - 0196-6553
IS - 11
ER -