Virus-Specific IgD in Acute Viral Infection of Mice

Demetrius Moskophidis, Matthäus Moskophidis, Jürgen Löhler

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

In phylogenetically diverse species with the help of T lymphocytes or soluble factors, viral infections induce the Ag-specific B lymphocytes to proliferate and terminally differentiate into IgM, IgG, IgA, IgD, or IgE Ab-secreting cells. Based on previous studies searching for IgD, it was inferred that serum IgD in the mouse is nearly undetectable, although in other species, e.g., humans, IgD is a measurable component of serum Ig. More recently, new information has been obtained indicating that IgD is secreted in minute quantities during normal B cell differentiation. We observed that IgD is secreted in significantly increased quantities in mice undergoing an acute infection with lymphocytic choriomeningitis virus or vesicular stomatitis virus compared with uninfected animals. A substantial fraction of the observed IgD was found to be virus specific. Using a solid-phase immunoenzymatic technique, virus-specific IgD Ab-forming cells were detected in the spleen; their numerical increase correlated with the level of secreted antiviral IgD. In addition, immunohistochemical staining revealed IgD+ plasma cells that occurred with a similar kinetic profile as the virus-specific IgD Ab-forming cells. These findings provide direct evidence that synthesis of IgD is a physiologic event in the mouse. Its precise function in the immune response to pathogens, however, remains to be determined.

Original languageEnglish (US)
Pages (from-to)1254-1261
Number of pages8
JournalJournal of Immunology
Volume158
Issue number3
StatePublished - Feb 1 1997

Fingerprint

Immunoglobulin D
Virus Diseases
Viruses
B-Lymphocytes
Lymphocytic choriomeningitis virus
Vesicular Stomatitis
Plasma Cells
Serum
Immunoglobulin A
Immunoglobulin E
Antiviral Agents
Immunoglobulin M
Cell Differentiation
Spleen

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Moskophidis, D., Moskophidis, M., & Löhler, J. (1997). Virus-Specific IgD in Acute Viral Infection of Mice. Journal of Immunology, 158(3), 1254-1261.

Virus-Specific IgD in Acute Viral Infection of Mice. / Moskophidis, Demetrius; Moskophidis, Matthäus; Löhler, Jürgen.

In: Journal of Immunology, Vol. 158, No. 3, 01.02.1997, p. 1254-1261.

Research output: Contribution to journalArticle

Moskophidis, D, Moskophidis, M & Löhler, J 1997, 'Virus-Specific IgD in Acute Viral Infection of Mice', Journal of Immunology, vol. 158, no. 3, pp. 1254-1261.
Moskophidis D, Moskophidis M, Löhler J. Virus-Specific IgD in Acute Viral Infection of Mice. Journal of Immunology. 1997 Feb 1;158(3):1254-1261.
Moskophidis, Demetrius ; Moskophidis, Matthäus ; Löhler, Jürgen. / Virus-Specific IgD in Acute Viral Infection of Mice. In: Journal of Immunology. 1997 ; Vol. 158, No. 3. pp. 1254-1261.
@article{d05e768253cf45eea38cd44b3409b5c3,
title = "Virus-Specific IgD in Acute Viral Infection of Mice",
abstract = "In phylogenetically diverse species with the help of T lymphocytes or soluble factors, viral infections induce the Ag-specific B lymphocytes to proliferate and terminally differentiate into IgM, IgG, IgA, IgD, or IgE Ab-secreting cells. Based on previous studies searching for IgD, it was inferred that serum IgD in the mouse is nearly undetectable, although in other species, e.g., humans, IgD is a measurable component of serum Ig. More recently, new information has been obtained indicating that IgD is secreted in minute quantities during normal B cell differentiation. We observed that IgD is secreted in significantly increased quantities in mice undergoing an acute infection with lymphocytic choriomeningitis virus or vesicular stomatitis virus compared with uninfected animals. A substantial fraction of the observed IgD was found to be virus specific. Using a solid-phase immunoenzymatic technique, virus-specific IgD Ab-forming cells were detected in the spleen; their numerical increase correlated with the level of secreted antiviral IgD. In addition, immunohistochemical staining revealed IgD+ plasma cells that occurred with a similar kinetic profile as the virus-specific IgD Ab-forming cells. These findings provide direct evidence that synthesis of IgD is a physiologic event in the mouse. Its precise function in the immune response to pathogens, however, remains to be determined.",
author = "Demetrius Moskophidis and Matth{\"a}us Moskophidis and J{\"u}rgen L{\"o}hler",
year = "1997",
month = "2",
day = "1",
language = "English (US)",
volume = "158",
pages = "1254--1261",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "3",

}

TY - JOUR

T1 - Virus-Specific IgD in Acute Viral Infection of Mice

AU - Moskophidis, Demetrius

AU - Moskophidis, Matthäus

AU - Löhler, Jürgen

PY - 1997/2/1

Y1 - 1997/2/1

N2 - In phylogenetically diverse species with the help of T lymphocytes or soluble factors, viral infections induce the Ag-specific B lymphocytes to proliferate and terminally differentiate into IgM, IgG, IgA, IgD, or IgE Ab-secreting cells. Based on previous studies searching for IgD, it was inferred that serum IgD in the mouse is nearly undetectable, although in other species, e.g., humans, IgD is a measurable component of serum Ig. More recently, new information has been obtained indicating that IgD is secreted in minute quantities during normal B cell differentiation. We observed that IgD is secreted in significantly increased quantities in mice undergoing an acute infection with lymphocytic choriomeningitis virus or vesicular stomatitis virus compared with uninfected animals. A substantial fraction of the observed IgD was found to be virus specific. Using a solid-phase immunoenzymatic technique, virus-specific IgD Ab-forming cells were detected in the spleen; their numerical increase correlated with the level of secreted antiviral IgD. In addition, immunohistochemical staining revealed IgD+ plasma cells that occurred with a similar kinetic profile as the virus-specific IgD Ab-forming cells. These findings provide direct evidence that synthesis of IgD is a physiologic event in the mouse. Its precise function in the immune response to pathogens, however, remains to be determined.

AB - In phylogenetically diverse species with the help of T lymphocytes or soluble factors, viral infections induce the Ag-specific B lymphocytes to proliferate and terminally differentiate into IgM, IgG, IgA, IgD, or IgE Ab-secreting cells. Based on previous studies searching for IgD, it was inferred that serum IgD in the mouse is nearly undetectable, although in other species, e.g., humans, IgD is a measurable component of serum Ig. More recently, new information has been obtained indicating that IgD is secreted in minute quantities during normal B cell differentiation. We observed that IgD is secreted in significantly increased quantities in mice undergoing an acute infection with lymphocytic choriomeningitis virus or vesicular stomatitis virus compared with uninfected animals. A substantial fraction of the observed IgD was found to be virus specific. Using a solid-phase immunoenzymatic technique, virus-specific IgD Ab-forming cells were detected in the spleen; their numerical increase correlated with the level of secreted antiviral IgD. In addition, immunohistochemical staining revealed IgD+ plasma cells that occurred with a similar kinetic profile as the virus-specific IgD Ab-forming cells. These findings provide direct evidence that synthesis of IgD is a physiologic event in the mouse. Its precise function in the immune response to pathogens, however, remains to be determined.

UR - http://www.scopus.com/inward/record.url?scp=0031065193&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031065193&partnerID=8YFLogxK

M3 - Article

C2 - 9013967

AN - SCOPUS:0031065193

VL - 158

SP - 1254

EP - 1261

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 3

ER -