Visual inspection versus quantitative flow cytometry to detect aberrant CD2 expression in malignant T cells

Indu Arun, Jacqueline A. Wulu, John E. Janik, Gregory A. Jasper, Constance M. Yuan, David Venzon, Maryalice Stetler-Stevenson

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background: Abnormal levels of T-cell antigen expression occur in T-cell neoplasia. We examined CD2 expression in malignant and normal T cells to determine if the level of CD2 expression differed significantly and if quantitation assisted in detecting this difference. Method: Flow cytometric immunophenotypic (FCI) evaluation was performed on specimens from 36 patients with mature T-cell neoplasia. Abnormal T cells were identified based upon the abnormal FCI and morphology. Levels of CD2 expression were quantitated using 1:1 PE conjugates of anti-CD2 and QuantiBRITE bead standards to calculate the antibodies bound per cell (ABC). The efficacy of ABC measurement versus simple examination of dots plots was compared. Results: Abnormal levels of CD2 expression were frequently observed in mature T-cell malignancies. The CD2 ABC values were highly sensitive in detecting differences between malignant and normal T cells (P 5 0.0028). In most cases (24/32 specimens, 75%), CD2 ABCs differed by >20%. CD2 ABCs had high variability in normal T cells. Conclusions: CD2 expression by malignant T cells differed significantly from that of normal T-cells by CD2 ABC quantitation. The high variability in normal T-cell CD2 ABCs limited the determination of normal reference ranges and, thus, its utility in the diagnosis of T-cell neoplasia. However, examination of CD2 can help in detection of tumor cells when residual normal T cells are present for comparison. Moreover, the increased sensitivity of CD2 quantitation is valuable in confirming FCI cases where abnormalities in CD2 expression are difficult to appreciate by visual inspection alone.

Original languageEnglish (US)
Pages (from-to)169-175
Number of pages7
JournalCytometry Part B - Clinical Cytometry
Volume78
Issue number3
DOIs
StatePublished - May 1 2010

Fingerprint

Flow Cytometry
T-Lymphocytes
Antibodies
Neoplasms
Reference Values
Viral Tumor Antigens

Keywords

  • ABC
  • CD2
  • Lymphoma
  • Normal T cells
  • Quantitation

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

Cite this

Arun, I., Wulu, J. A., Janik, J. E., Jasper, G. A., Yuan, C. M., Venzon, D., & Stetler-Stevenson, M. (2010). Visual inspection versus quantitative flow cytometry to detect aberrant CD2 expression in malignant T cells. Cytometry Part B - Clinical Cytometry, 78(3), 169-175. https://doi.org/10.1002/cyto.b.20507

Visual inspection versus quantitative flow cytometry to detect aberrant CD2 expression in malignant T cells. / Arun, Indu; Wulu, Jacqueline A.; Janik, John E.; Jasper, Gregory A.; Yuan, Constance M.; Venzon, David; Stetler-Stevenson, Maryalice.

In: Cytometry Part B - Clinical Cytometry, Vol. 78, No. 3, 01.05.2010, p. 169-175.

Research output: Contribution to journalArticle

Arun, I, Wulu, JA, Janik, JE, Jasper, GA, Yuan, CM, Venzon, D & Stetler-Stevenson, M 2010, 'Visual inspection versus quantitative flow cytometry to detect aberrant CD2 expression in malignant T cells', Cytometry Part B - Clinical Cytometry, vol. 78, no. 3, pp. 169-175. https://doi.org/10.1002/cyto.b.20507
Arun, Indu ; Wulu, Jacqueline A. ; Janik, John E. ; Jasper, Gregory A. ; Yuan, Constance M. ; Venzon, David ; Stetler-Stevenson, Maryalice. / Visual inspection versus quantitative flow cytometry to detect aberrant CD2 expression in malignant T cells. In: Cytometry Part B - Clinical Cytometry. 2010 ; Vol. 78, No. 3. pp. 169-175.
@article{505f24636a604bdd9066e0d82bd488e0,
title = "Visual inspection versus quantitative flow cytometry to detect aberrant CD2 expression in malignant T cells",
abstract = "Background: Abnormal levels of T-cell antigen expression occur in T-cell neoplasia. We examined CD2 expression in malignant and normal T cells to determine if the level of CD2 expression differed significantly and if quantitation assisted in detecting this difference. Method: Flow cytometric immunophenotypic (FCI) evaluation was performed on specimens from 36 patients with mature T-cell neoplasia. Abnormal T cells were identified based upon the abnormal FCI and morphology. Levels of CD2 expression were quantitated using 1:1 PE conjugates of anti-CD2 and QuantiBRITE bead standards to calculate the antibodies bound per cell (ABC). The efficacy of ABC measurement versus simple examination of dots plots was compared. Results: Abnormal levels of CD2 expression were frequently observed in mature T-cell malignancies. The CD2 ABC values were highly sensitive in detecting differences between malignant and normal T cells (P 5 0.0028). In most cases (24/32 specimens, 75{\%}), CD2 ABCs differed by >20{\%}. CD2 ABCs had high variability in normal T cells. Conclusions: CD2 expression by malignant T cells differed significantly from that of normal T-cells by CD2 ABC quantitation. The high variability in normal T-cell CD2 ABCs limited the determination of normal reference ranges and, thus, its utility in the diagnosis of T-cell neoplasia. However, examination of CD2 can help in detection of tumor cells when residual normal T cells are present for comparison. Moreover, the increased sensitivity of CD2 quantitation is valuable in confirming FCI cases where abnormalities in CD2 expression are difficult to appreciate by visual inspection alone.",
keywords = "ABC, CD2, Lymphoma, Normal T cells, Quantitation",
author = "Indu Arun and Wulu, {Jacqueline A.} and Janik, {John E.} and Jasper, {Gregory A.} and Yuan, {Constance M.} and David Venzon and Maryalice Stetler-Stevenson",
year = "2010",
month = "5",
day = "1",
doi = "10.1002/cyto.b.20507",
language = "English (US)",
volume = "78",
pages = "169--175",
journal = "Cytometry",
issn = "0196-4763",
publisher = "John Wiley and Sons Inc.",
number = "3",

}

TY - JOUR

T1 - Visual inspection versus quantitative flow cytometry to detect aberrant CD2 expression in malignant T cells

AU - Arun, Indu

AU - Wulu, Jacqueline A.

AU - Janik, John E.

AU - Jasper, Gregory A.

AU - Yuan, Constance M.

AU - Venzon, David

AU - Stetler-Stevenson, Maryalice

PY - 2010/5/1

Y1 - 2010/5/1

N2 - Background: Abnormal levels of T-cell antigen expression occur in T-cell neoplasia. We examined CD2 expression in malignant and normal T cells to determine if the level of CD2 expression differed significantly and if quantitation assisted in detecting this difference. Method: Flow cytometric immunophenotypic (FCI) evaluation was performed on specimens from 36 patients with mature T-cell neoplasia. Abnormal T cells were identified based upon the abnormal FCI and morphology. Levels of CD2 expression were quantitated using 1:1 PE conjugates of anti-CD2 and QuantiBRITE bead standards to calculate the antibodies bound per cell (ABC). The efficacy of ABC measurement versus simple examination of dots plots was compared. Results: Abnormal levels of CD2 expression were frequently observed in mature T-cell malignancies. The CD2 ABC values were highly sensitive in detecting differences between malignant and normal T cells (P 5 0.0028). In most cases (24/32 specimens, 75%), CD2 ABCs differed by >20%. CD2 ABCs had high variability in normal T cells. Conclusions: CD2 expression by malignant T cells differed significantly from that of normal T-cells by CD2 ABC quantitation. The high variability in normal T-cell CD2 ABCs limited the determination of normal reference ranges and, thus, its utility in the diagnosis of T-cell neoplasia. However, examination of CD2 can help in detection of tumor cells when residual normal T cells are present for comparison. Moreover, the increased sensitivity of CD2 quantitation is valuable in confirming FCI cases where abnormalities in CD2 expression are difficult to appreciate by visual inspection alone.

AB - Background: Abnormal levels of T-cell antigen expression occur in T-cell neoplasia. We examined CD2 expression in malignant and normal T cells to determine if the level of CD2 expression differed significantly and if quantitation assisted in detecting this difference. Method: Flow cytometric immunophenotypic (FCI) evaluation was performed on specimens from 36 patients with mature T-cell neoplasia. Abnormal T cells were identified based upon the abnormal FCI and morphology. Levels of CD2 expression were quantitated using 1:1 PE conjugates of anti-CD2 and QuantiBRITE bead standards to calculate the antibodies bound per cell (ABC). The efficacy of ABC measurement versus simple examination of dots plots was compared. Results: Abnormal levels of CD2 expression were frequently observed in mature T-cell malignancies. The CD2 ABC values were highly sensitive in detecting differences between malignant and normal T cells (P 5 0.0028). In most cases (24/32 specimens, 75%), CD2 ABCs differed by >20%. CD2 ABCs had high variability in normal T cells. Conclusions: CD2 expression by malignant T cells differed significantly from that of normal T-cells by CD2 ABC quantitation. The high variability in normal T-cell CD2 ABCs limited the determination of normal reference ranges and, thus, its utility in the diagnosis of T-cell neoplasia. However, examination of CD2 can help in detection of tumor cells when residual normal T cells are present for comparison. Moreover, the increased sensitivity of CD2 quantitation is valuable in confirming FCI cases where abnormalities in CD2 expression are difficult to appreciate by visual inspection alone.

KW - ABC

KW - CD2

KW - Lymphoma

KW - Normal T cells

KW - Quantitation

UR - http://www.scopus.com/inward/record.url?scp=77952590983&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77952590983&partnerID=8YFLogxK

U2 - 10.1002/cyto.b.20507

DO - 10.1002/cyto.b.20507

M3 - Article

C2 - 20020522

AN - SCOPUS:77952590983

VL - 78

SP - 169

EP - 175

JO - Cytometry

JF - Cytometry

SN - 0196-4763

IS - 3

ER -