Visual sensitivity and interphotoreceptor retinoid binding protein in the mouse: Regulation by vitamin A

Gregory I. Liou, Suraporn Matragoon, De Mao Chen, Chun Lan Gao, Lu Zhang, Yijian Fei, Martin L. Katz, William S. Stark

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Interphotoreceptor retinoid binding protein (IRBP) is a retinoid and fatty acid binding glycoprotein secreted by rod and cone photoreceptors in all vertebrates. IRBP is believed to serve as a carrier for retinoids in the bleaching and regeneration cycle of rhodopsin. IRBP protein has been found to be decreased in vitamin A-deprived rats; it is rapidly recovered after retinol repletion. To understand the mechanism for this recovery, we determined whether vitamin A affects transcription and translation of the IRBP gene. Wild-type and transgenic mice harboring the IRBP promoter-CAT reporter fusion gene were maintained on a retinol-deficient diet supplemented with retinoic acid (-A) or on a control diet (+A) for up to 60 wk postweaning. Some of the -A mice were given retinol repletion for 7 days (- A+A). Electroretinography analysis revealed alterations in waveform and a 2 log unit decrease in b-wave sensitivity in the -A mice over a broad range of stimulus wavelengths. Retinol repletion effected a full recovery. Immunochemistry showed a significant decrease in the immunogold-labeled IRBP between the retinal pigment epithelium and the outer segments of the -A mice compared with +A and -A+A mice. Northern blots showed no differences in the amounts of IRBP or CAT mRNA between these three treatment groups. These results suggest that the regulation of IRBP by retinol is not transcriptional.

Original languageEnglish (US)
Pages (from-to)129-138
Number of pages10
JournalFASEB Journal
Volume12
Issue number1
StatePublished - Feb 13 1998

Fingerprint

Vitamin A
Retinoids
Nutrition
Genes
Diet
Retinal Cone Photoreceptor Cells
Retinal Rod Photoreceptor Cells
Electroretinography
Immunochemistry
Recovery
Vertebrate Photoreceptor Cells
Retinal Pigments
Rhodopsin
interstitial retinol-binding protein
Retinal Pigment Epithelium
Transcription
Bleaching
Tretinoin
Reporter Genes
Northern Blotting

Keywords

  • Electroretinography
  • IRBP
  • Photoreceptor
  • Vitamin A (retinol) deficiency and repletion

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Liou, G. I., Matragoon, S., Chen, D. M., Gao, C. L., Zhang, L., Fei, Y., ... Stark, W. S. (1998). Visual sensitivity and interphotoreceptor retinoid binding protein in the mouse: Regulation by vitamin A. FASEB Journal, 12(1), 129-138.

Visual sensitivity and interphotoreceptor retinoid binding protein in the mouse : Regulation by vitamin A. / Liou, Gregory I.; Matragoon, Suraporn; Chen, De Mao; Gao, Chun Lan; Zhang, Lu; Fei, Yijian; Katz, Martin L.; Stark, William S.

In: FASEB Journal, Vol. 12, No. 1, 13.02.1998, p. 129-138.

Research output: Contribution to journalArticle

Liou, GI, Matragoon, S, Chen, DM, Gao, CL, Zhang, L, Fei, Y, Katz, ML & Stark, WS 1998, 'Visual sensitivity and interphotoreceptor retinoid binding protein in the mouse: Regulation by vitamin A', FASEB Journal, vol. 12, no. 1, pp. 129-138.
Liou GI, Matragoon S, Chen DM, Gao CL, Zhang L, Fei Y et al. Visual sensitivity and interphotoreceptor retinoid binding protein in the mouse: Regulation by vitamin A. FASEB Journal. 1998 Feb 13;12(1):129-138.
Liou, Gregory I. ; Matragoon, Suraporn ; Chen, De Mao ; Gao, Chun Lan ; Zhang, Lu ; Fei, Yijian ; Katz, Martin L. ; Stark, William S. / Visual sensitivity and interphotoreceptor retinoid binding protein in the mouse : Regulation by vitamin A. In: FASEB Journal. 1998 ; Vol. 12, No. 1. pp. 129-138.
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