Warmer preparation of hippocampal slices prevents synapse proliferation that might obscure LTP-related structural plasticity

Jennifer N. Bourne, Sergei A Kirov, Karin E. Sorra, Kristen M. Harris

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

The hippocampal slice is a popular model system in which to study the cellular properties of long-term potentiation (LTP). Synaptogenesis induced by exposure to ice-cold artificial cerebrospinal fluid (ACSF), however, raises the concern that morphological correlates of LTP might be obscured, especially in mature slices. Here we demonstrate that preparation of mature hippocampal slices at room temperature (∼25 °C) maintains excellent ultrastructure and a synapse density comparable to perfusion-fixed hippocampus. These results suggest that slices prepared at room temperature might provide a better basis from which to detect LTP-related changes in synapse number and morphology.

Original languageEnglish (US)
Pages (from-to)55-59
Number of pages5
JournalNeuropharmacology
Volume52
Issue number1
DOIs
StatePublished - Jan 1 2007

Keywords

  • Adult
  • Dendritic spine
  • Hippocampal slice
  • Ultrastructure

ASJC Scopus subject areas

  • Pharmacology
  • Cellular and Molecular Neuroscience

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