Whole-genome transcriptional profiles of a novel marine fish iridovirus, Singapore grouper iridovirus (SGIV) in virus-infected grouper spleen cell cultures and in orange-spotted grouper, Epinephulus coioides

Yong Teng, Zhiwei Hou, Jie Gong, Hong Liu, Xiayang Xie, Liang Zhang, Xiaohong Chen, Qi Wei Qin

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

A DNA microarray containing all Singapore grouper iridovirus (SGIV) open reading frames (ORFs) was constructed to map the viral gene transcriptional profiles in virus-infected grouper spleen (GS) cells and in spleen tissues of virus-infected grouper. The results showed that viral genes started to be transcribed as early as 1 h postinfection (p.i.), and followed by a rapid increasing gene expression along with virus infection in cell cultures. The three temporal kinetic classes (15 immediate-early, 89 early and 53 late transcripts) were classified during an in vitro infection by their dependence on de novo protein synthesis and viral DNA replication inhibitors. In SGIV-infected grouper, Epinephulus coioides, most of the viral genes were expressed between 1 and 4 d p.i., and the number and expression levels started to decrease after 5 d p.i. These data were confirmed by real-time RT-PCR. This study provides an experimental basis for investigation of virus-host interactions and the development of control strategies against SGIV infection.

Original languageEnglish (US)
Pages (from-to)39-48
Number of pages10
JournalVirology
Volume377
Issue number1
DOIs
StatePublished - Jul 20 2008
Externally publishedYes

Fingerprint

Iridovirus
Viral Genes
Singapore
Fishes
Spleen
Cell Culture Techniques
Genome
Viruses
Viral DNA
Virus Diseases
Infection
Oligonucleotide Array Sequence Analysis
DNA Replication
Open Reading Frames
Real-Time Polymerase Chain Reaction
Gene Expression
Proteins

Keywords

  • Expression pattern
  • Grouper
  • SGIV
  • Temporal kinetic class
  • Transcriptional profiles
  • cDNA microarrays

ASJC Scopus subject areas

  • Virology

Cite this

Whole-genome transcriptional profiles of a novel marine fish iridovirus, Singapore grouper iridovirus (SGIV) in virus-infected grouper spleen cell cultures and in orange-spotted grouper, Epinephulus coioides. / Teng, Yong; Hou, Zhiwei; Gong, Jie; Liu, Hong; Xie, Xiayang; Zhang, Liang; Chen, Xiaohong; Qin, Qi Wei.

In: Virology, Vol. 377, No. 1, 20.07.2008, p. 39-48.

Research output: Contribution to journalArticle

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