Whole isolated neocortical and hippocampal preparations and their use in imaging studies

Melissa L. Davies, Sergei A. Kirov, R. David Andrew

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

This study shows that two whole isolated preparations from the young mouse, the neocortical 'slab' and the hippocampal formation, are useful for imaging studies requiring both global monitoring using light transmittance (LT) imaging and high resolution cellular monitoring using 2-photon laser scanning microscopy (2PLSM). These preparations share advantages with brain slices such as maintaining intrinsic neuronal properties and avoiding cardiac or respiratory movement. Important additional advantages include the maintenance of all local input and output pathways, the absence of surfaces injured by slicing and the preservation of three-dimensional tissue structure. Using evoked extracellular field recording, we demonstrate long-term (hours) viability of both whole preparations. We then show that propagating cortical events such as anoxic depolarization (AD) and spreading depression (SD) can be imaged in both preparations, yielding results comparable to those in brain slices but retaining the tissue's three-dimensional structure. Using transgenic mice expressing green fluorescent protein (GFP) in pyramidal and granule cell neurons, 2PLSM confirms that these preparations are free of the surface damage observed in sliced brain tissue. Moreover the neurons undergo swelling with accompanying dendritic beading following AD induced by simulated ischemia, similar to cortical damage described in vivo.

Original languageEnglish (US)
Pages (from-to)203-216
Number of pages14
JournalJournal of Neuroscience Methods
Volume166
Issue number2
DOIs
StatePublished - Nov 30 2007

Keywords

  • 2-Photon microscopy
  • Anoxic depolarization
  • GFP
  • Ischemia
  • Light transmittance
  • Spreading depression
  • Stroke

ASJC Scopus subject areas

  • Neuroscience(all)

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