STRUCTURE OF DNA--SPECTROSCOPIC STUDIES

Project: Research project

Project Details

Description

The three dimensional structure of DNA as influenced by base sequence,
metal ions, chemical carcinogens and other agents will be studied by
vacuum-ultraviolet circular dichroism (VUV-CD) and time resolved
fluorescence. The electronic states and transitions of the DNA bases, understanding of
which is crucial to the interpretation of CD spectra, will be studied using
vacuum-ultraviolet magnetic circular dichroism (VUV-MCD) and fluorescence
polarization spectroscopy. A major goal is understanding why VUV-CD is a better indicator of the
helical structure of, and more sensitive to the hydrogen bonding between
DNA strands than is conventional, longer wavelength CD. The VUV-CD, VUV-MCD, fluorescence polarization and time-resolved
fluorescence experiments will be performed at the National Synchrotron
Light Source.
StatusNot started