α-Actinin-2, a cytoskeletal protein, binds to angiogenin

Huajun Hu, Xiangwei Gao, Yishan Sun, Jiliang Zhou, Min Yang, Zhengping Xu

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Angiogenin is an angiogenic factor which is involved in tumorigenesis. However, no particular intracellular protein is known to interact directly with angiogenin. In the present study, we reported the identification of α-actinin-2, an actin-crosslinking protein, as a potential angiogenin-interacting partner by yeast two-hybrid screening. This interaction was confirmed by different approaches. First, angiogenin was pulled down together with His-tagged α-actinin-2 by Ni2+-agarose resins. Second, α-actinin-2 was coimmunoprecipitated with angiogenin by anti-angiogenin monoclonal antibody. Third, the in vivo interaction of these two proteins was revealed by fluorescence resonance energy transfer analysis. Since members of α-actinin family play pivotal roles in cell proliferation, migration, and invasion, the interaction between α-actinin-2 and angiogenin may underline one possible mechanism of angiogenin in angiogenesis. Our finding presents the first evidence of an interaction of a cytosolic protein with angiogenin, which might be a novel interference target for anti-angiogenesis and anti-tumor therapy.

Original languageEnglish (US)
Pages (from-to)661-667
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume329
Issue number2
DOIs
Publication statusPublished - Apr 8 2005
Externally publishedYes

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Keywords

  • Angiogenesis
  • Angiogenin
  • Protein interaction
  • α-Actinin-2

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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