γ-Glutamyl transpeptidase has a role in the persistent colonization of the avian gut by Campylobacter jejuni

If H.A. Barnes, Mary C. Bagnall, Darren D. Browning, Stuart A. Thompson, Georgina Manning, Diane G. Newell

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

The contribution of γ-glutamyl transpeptidase (GGT) to Campylobacter jejuni virulence and colonization of the avian gut has been investigated. The presence of the ggt gene in C. jejuni strains directly correlated with the expression of GGT activity as measured by cleavage and transfer of the γ-glutamyl moiety. Inactivation of the monocistronic ggt gene in C. jejuni strain 81116 resulted in isogenic mutants with undetectable GGT activity; nevertheless, these mutants grew normally in vitro. However, the mutants had increased motility, a 5.4-fold higher invasion efficiency into INT407 cells in vitro and increased resistance to hydrogen peroxide stress. Moreover, the apoptosis-inducing activity of the ggt mutant was significantly lower than that of the parental strain. In vivo studies showed that, although GGT activity was not required for initial colonization of 1-day-old chicks, the enzyme was required for persistant colonization of the avian gut.

Original languageEnglish (US)
Pages (from-to)198-207
Number of pages10
JournalMicrobial Pathogenesis
Volume43
Issue number5-6
DOIs
StatePublished - Nov 1 2007

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Campylobacter jejuni
gamma-Glutamyltransferase
Hydrogen Peroxide
Genes
Virulence
Apoptosis
Enzymes
In Vitro Techniques

Keywords

  • Avian colonization
  • Campylobacter jejuni
  • γ-glutamyl transpeptidase (ggt)

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

Cite this

γ-Glutamyl transpeptidase has a role in the persistent colonization of the avian gut by Campylobacter jejuni. / Barnes, If H.A.; Bagnall, Mary C.; Browning, Darren D.; Thompson, Stuart A.; Manning, Georgina; Newell, Diane G.

In: Microbial Pathogenesis, Vol. 43, No. 5-6, 01.11.2007, p. 198-207.

Research output: Contribution to journalArticle

Barnes, If H.A. ; Bagnall, Mary C. ; Browning, Darren D. ; Thompson, Stuart A. ; Manning, Georgina ; Newell, Diane G. / γ-Glutamyl transpeptidase has a role in the persistent colonization of the avian gut by Campylobacter jejuni. In: Microbial Pathogenesis. 2007 ; Vol. 43, No. 5-6. pp. 198-207.
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