δ-Bag cell peptide from the egg-laying hormone precursor of Aplysia. Processing, primary structure, and biological activity

Gregg Thomas Nagle, M. De Jong-Brink, S. D. Painter, M. M.J. Bergamin-Sassen, J. E. Blankenship, A. Kurosky

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The bag cells of the marine mollusk Aplysia express a gene encoding a 271-residue egg-laying hormone (ELH) precursor that is processed into at least nine peptide products. Four of the peptides have been identified in bag cell releasates and are known to act as nonsynaptic neurotransmitters in the abdominal ganglion. The isolation, primary structure, and proposed biological activity of a fifth peptide product [δ-bag cell peptide (δ-BCP)] from the ELH precursor are described. δ-BCP was established to be a 39-residue peptide: NH2-Asp-Gln-Asp-Glu-Gly-Asn-Phe-Arg-Arg-Phe-Pro-Thr-Asn-Ala-Val-Ser-Met- Ser-Ala-Asp-Glu-Asn-Ser-Pro-Phe-Asp-Leu-Ser-Asn-Glu-Asp-Gly-Ala-Val-Tyr- Gln-Arg-Asp-Leu-COOH. This sequence corresponds to residues 81-119 of the ELH prohormone and shares sequence identity with atrial gland peptides A and B. Significantly, synthetic δ-BCP stimulated Ca2+ uptake into mitochondria of secretory cells in the albumin gland in vitro, suggesting that the peptide regulates the cellular release of perivitelline fluid by the gland. Similar results were obtained with purified peptide A and a shorter version of δ-BCP [δ-BCP-(14-33)]. These results indicate that δ-BCP belongs to a family of structurally related peptides with similar pharmacological activities that center at a conserved region of sequence corresponding to δ-BCP-(14-33).

Original languageEnglish (US)
Pages (from-to)22329-22335
Number of pages7
JournalJournal of Biological Chemistry
Volume265
Issue number36
StatePublished - Dec 1 1990
Externally publishedYes

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Bioactivity
Hormones
Peptides
Processing
Ovum
Aplysia
Mitochondria
Gene encoding
Conserved Sequence
Mollusca
Ganglia
Neurotransmitter Agents
Mollusca egg-laying hormone
Albumins
Pharmacology
Fluids
Genes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Nagle, G. T., De Jong-Brink, M., Painter, S. D., Bergamin-Sassen, M. M. J., Blankenship, J. E., & Kurosky, A. (1990). δ-Bag cell peptide from the egg-laying hormone precursor of Aplysia. Processing, primary structure, and biological activity. Journal of Biological Chemistry, 265(36), 22329-22335.

δ-Bag cell peptide from the egg-laying hormone precursor of Aplysia. Processing, primary structure, and biological activity. / Nagle, Gregg Thomas; De Jong-Brink, M.; Painter, S. D.; Bergamin-Sassen, M. M.J.; Blankenship, J. E.; Kurosky, A.

In: Journal of Biological Chemistry, Vol. 265, No. 36, 01.12.1990, p. 22329-22335.

Research output: Contribution to journalArticle

Nagle, GT, De Jong-Brink, M, Painter, SD, Bergamin-Sassen, MMJ, Blankenship, JE & Kurosky, A 1990, 'δ-Bag cell peptide from the egg-laying hormone precursor of Aplysia. Processing, primary structure, and biological activity', Journal of Biological Chemistry, vol. 265, no. 36, pp. 22329-22335.
Nagle GT, De Jong-Brink M, Painter SD, Bergamin-Sassen MMJ, Blankenship JE, Kurosky A. δ-Bag cell peptide from the egg-laying hormone precursor of Aplysia. Processing, primary structure, and biological activity. Journal of Biological Chemistry. 1990 Dec 1;265(36):22329-22335.
Nagle, Gregg Thomas ; De Jong-Brink, M. ; Painter, S. D. ; Bergamin-Sassen, M. M.J. ; Blankenship, J. E. ; Kurosky, A. / δ-Bag cell peptide from the egg-laying hormone precursor of Aplysia. Processing, primary structure, and biological activity. In: Journal of Biological Chemistry. 1990 ; Vol. 265, No. 36. pp. 22329-22335.
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