[10] Gangliosides: Structure, Isolation, and Analysis

Robert W. Ledeen, Robert K Yu

Research output: Contribution to journalArticle

645 Citations (Scopus)

Abstract

This chapter discusses structure and procedure for isolation, and analysis of gangliosides. Most widely used procedure for isolation is based on the ability of gangliosides to partition from the chloroform-methanol phase in which they are extracted into the upper water-enriched phase, leaving behind the bulk of other lipids. There is also a procedure based on DEAE-Sephadex. This method employs an anion-exchange resin to separate gangliosides first, along with other acidic lipids, from the much larger quantity of neutral and zwitterionic lipids that do not bind to the resin. The subsequent steps of base treatment, dialysis, and silicic acid chromatography are designed to remove the other acidic lipids and any acidic low molecular weight contaminants that accompany the gangliosides. Other types of chromatography is used for resolution. Quantitative estimation of gangliosides is usually based on the sialic acid moiety. Various procedures are outlined, along with a gas-liquid chromatographic method that has the attributes, of specificity and sensitivity.

Original languageEnglish (US)
Pages (from-to)139-191
Number of pages53
JournalMethods in Enzymology
Volume83
Issue numberC
DOIs
StatePublished - Jan 1 1982

Fingerprint

Gangliosides
Lipids
Chromatography
Silicic Acid
Anion Exchange Resins
DEAE-Dextran
Dialysis
N-Acetylneuraminic Acid
Chloroform
Methanol
Resins
Gases
Molecular weight
Impurities
Molecular Weight
Water
Liquids
Sensitivity and Specificity

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

[10] Gangliosides : Structure, Isolation, and Analysis. / Ledeen, Robert W.; Yu, Robert K.

In: Methods in Enzymology, Vol. 83, No. C, 01.01.1982, p. 139-191.

Research output: Contribution to journalArticle

Ledeen, Robert W. ; Yu, Robert K. / [10] Gangliosides : Structure, Isolation, and Analysis. In: Methods in Enzymology. 1982 ; Vol. 83, No. C. pp. 139-191.
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