This chapter discusses structure and procedure for isolation, and analysis of gangliosides. Most widely used procedure for isolation is based on the ability of gangliosides to partition from the chloroform-methanol phase in which they are extracted into the upper water-enriched phase, leaving behind the bulk of other lipids. There is also a procedure based on DEAE-Sephadex. This method employs an anion-exchange resin to separate gangliosides first, along with other acidic lipids, from the much larger quantity of neutral and zwitterionic lipids that do not bind to the resin. The subsequent steps of base treatment, dialysis, and silicic acid chromatography are designed to remove the other acidic lipids and any acidic low molecular weight contaminants that accompany the gangliosides. Other types of chromatography is used for resolution. Quantitative estimation of gangliosides is usually based on the sialic acid moiety. Various procedures are outlined, along with a gas-liquid chromatographic method that has the attributes, of specificity and sensitivity.
ASJC Scopus subject areas
- Molecular Biology