TY - JOUR
T1 - 1,25-Dihydroxyvitamin D3 induces phospholipase D-1 expression in primary mouse epidermal keratinocytes
AU - Griner, Richard D.
AU - Qin, Feng
AU - Jung, Eun Mi
AU - Sue-Ling, Christopher K.
AU - Crawford, Kimberly B.
AU - Mann-Blakeney, Ra Shawn
AU - Bollag, Roni J.
AU - Bollag, Wendy Bollinger
PY - 1999/2/19
Y1 - 1999/2/19
N2 - The hormone 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) elicits the programmed pattern of differentiation in epidermal keratinocytes. Based on data indicating a potential role of phospholipase D (PLD) in mediating keratinocyte differentiation, we investigated the effect of 1,25(OH)2D3 on PLD expression. A 24-h exposure to 1,25(OH)2D3 stimulated PLD-1, but not PLD-2, mRNA expression. This 1,25(OH)2D3-enhanced expression was accompanied by increased total PLD and PLD-1 activity. Time course studies indicated that 1,25(OH)2D3 induced PLD-1 expression by 8 h, with a maximal increase at 20-24 h. Exposure to 1,25(OH)2D3 inhibited proliferation over the same time period with similar kinetics. Expression of the early (spinous) differentiation marker keratin 1 decreased in response to 1,25(OH)2D3 over 12-24 h. Treatment with 1,25(OH)2D3 enhanced the activity of transglutaminase, a late (granular) differentiation marker, by 12 h with a maximal increase after 24 h. In situ hybridization studies demonstrated that the highest levels of PLD-1 expression are in the more differentiated (spinous and granular) layers of the epidermis, with little expression in basal keratinocytes. Our results suggest a role for PLD expression/activity during keratinocyte differentiation.
AB - The hormone 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) elicits the programmed pattern of differentiation in epidermal keratinocytes. Based on data indicating a potential role of phospholipase D (PLD) in mediating keratinocyte differentiation, we investigated the effect of 1,25(OH)2D3 on PLD expression. A 24-h exposure to 1,25(OH)2D3 stimulated PLD-1, but not PLD-2, mRNA expression. This 1,25(OH)2D3-enhanced expression was accompanied by increased total PLD and PLD-1 activity. Time course studies indicated that 1,25(OH)2D3 induced PLD-1 expression by 8 h, with a maximal increase at 20-24 h. Exposure to 1,25(OH)2D3 inhibited proliferation over the same time period with similar kinetics. Expression of the early (spinous) differentiation marker keratin 1 decreased in response to 1,25(OH)2D3 over 12-24 h. Treatment with 1,25(OH)2D3 enhanced the activity of transglutaminase, a late (granular) differentiation marker, by 12 h with a maximal increase after 24 h. In situ hybridization studies demonstrated that the highest levels of PLD-1 expression are in the more differentiated (spinous and granular) layers of the epidermis, with little expression in basal keratinocytes. Our results suggest a role for PLD expression/activity during keratinocyte differentiation.
UR - http://www.scopus.com/inward/record.url?scp=0033582519&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033582519&partnerID=8YFLogxK
U2 - 10.1074/jbc.274.8.4663
DO - 10.1074/jbc.274.8.4663
M3 - Article
C2 - 9988703
AN - SCOPUS:0033582519
SN - 0021-9258
VL - 274
SP - 4663
EP - 4670
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 8
ER -