1,8-Cineole (eucalyptol) mitigates inflammation in amyloid beta toxicated PC12 cells: Relevance to alzheimer's disease

Fakhrul Islam, Andleeb Khan, Kumar Vaibhav, Hayate Javed, Rizwana Tabassum, Md Ejaz Ahmed, Mohd Moshahid Khan, Mohammad Badruzzaman Khan, Pallavi Shrivastava, Farah Islam, M. Saeed Siddiqui, M. M. Safhi

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

Inflammatory process has a fundamental role in the pathogenesis of Alzheimer's disease and insoluble amyloid beta deposits and neurofibrillary tangles provide the obvious stimuli for inflammation. The present study demonstrate the effect of pretreatment of 1,8-cineole (Cin) on inflammation induced by Aβ(25-35) in differentiated PC12 cells. The cells were treated with Cin at different doses for 24 h and then replaced by media containing Aβ(25-35) for another 24 h. The cell viability was decreased in Aβ(25-35) treated cells which was significantly restored by Cin pretreatment. Cin successfully reduced the mitochondrial membrane potential, ROS and NO levels in Aβ(25-35) treated cells. Cin also lowered the levels of proinflammatory cytokines TNF-α, IL-1β and IL-6 in Aβ(25-35) treated cells. Moreover, Cin also succeeded in lowering the expression of NOS-2, COX-2 and NF-κB. This study suggests the protective effects of Cin on inflammation and provides additional evidence for its potential beneficial use in therapy as an anti-inflammatory agent in neurodegenerative disease.

Original languageEnglish (US)
Pages (from-to)344-352
Number of pages9
JournalNeurochemical Research
Volume39
Issue number2
DOIs
Publication statusPublished - Feb 1 2014

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Keywords

  • Amyloid beta (Aβ)
  • COX-2
  • Cineole
  • Cytokines
  • NF-κB
  • NOS-2

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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