20-Hydroxyeicosatetraenoic Acid (20-HETE) Metabolism in Coronary Endothelial Cells

Terry L. Kaduce, Xiang Fang, Shawn D. Harmon, Christine L. Oltman, Kevin C Dellsperger, Lynn M. Teesch, V. Raj Gopal, J. R. Falck, William B. Campbell, Neal Lee Weintraub, Arthur A. Spector

Research output: Contribution to journalArticle

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Abstract

We have investigated the role of endothelial cells in the metabolism of 20-hydroxyeicosatetraenoic acid (20-HETE), a vasoactive mediator synthesized from arachidonic acid by cytochrome P450 ω-oxidases. Porcine coronary artery endothelial cells (PCEC) incorporated 20-[3H]HETE primarily into the sn-2 position of phospholipids through a coenzyme A-dependent process. The incorporation was reduced by equimolar amounts of arachidonic, eicosapentaenoic or 8,9-epoxyeicosatrienoic acids, but some uptake persisted even when a 10-fold excess of arachidonic acid was available. The retention of 20-[3H]HETE increased substantially when methyl arachidonoyl fluorophosphonate, but not bromoenol lactone, was added, suggesting that a Ca2+-dependent cytosolic phospholipase A2 released the 20-HETE contained in PCEC phospholipids. Addition of calcium ionophore A23187 produced a rapid release of 20-[3H]HETE from the PCEC, a finding that also is consistent with a Ca2+-dependent mobilization process. PCEC also converted 20-[3H]HETE to 20-carboxy-arachidonic acid (20-COOH-AA) and 18-, 16-, and 14-carbon β-oxidation products. 20-COOH-AA produced vasodilation in porcine coronary arterioles, but 20-HETE was inactive. These results suggest that the incorporation of 20-HETE and its subsequent conversion to 20-COOH-AA in the endothelium may be important in modulating coronary vascular function.

Original languageEnglish (US)
Pages (from-to)2648-2656
Number of pages9
JournalJournal of Biological Chemistry
Volume279
Issue number4
DOIs
StatePublished - Jan 23 2004

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Endothelial cells
Hydroxyeicosatetraenoic Acids
Metabolism
Endothelial Cells
Arachidonic Acid
Swine
Coronary Vessels
Phospholipids
Cytosolic Phospholipases A2
Calcium Ionophores
Coenzyme A
Cytochrome P-450 Enzyme System
Oxidoreductases
Carbon
20-hydroxy-5,8,11,14-eicosatetraenoic acid
Calcimycin
Arterioles
Electron Transport Complex IV
Oxidation
Vasodilation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Kaduce, T. L., Fang, X., Harmon, S. D., Oltman, C. L., Dellsperger, K. C., Teesch, L. M., ... Spector, A. A. (2004). 20-Hydroxyeicosatetraenoic Acid (20-HETE) Metabolism in Coronary Endothelial Cells. Journal of Biological Chemistry, 279(4), 2648-2656. https://doi.org/10.1074/jbc.M306849200

20-Hydroxyeicosatetraenoic Acid (20-HETE) Metabolism in Coronary Endothelial Cells. / Kaduce, Terry L.; Fang, Xiang; Harmon, Shawn D.; Oltman, Christine L.; Dellsperger, Kevin C; Teesch, Lynn M.; Gopal, V. Raj; Falck, J. R.; Campbell, William B.; Weintraub, Neal Lee; Spector, Arthur A.

In: Journal of Biological Chemistry, Vol. 279, No. 4, 23.01.2004, p. 2648-2656.

Research output: Contribution to journalArticle

Kaduce, TL, Fang, X, Harmon, SD, Oltman, CL, Dellsperger, KC, Teesch, LM, Gopal, VR, Falck, JR, Campbell, WB, Weintraub, NL & Spector, AA 2004, '20-Hydroxyeicosatetraenoic Acid (20-HETE) Metabolism in Coronary Endothelial Cells', Journal of Biological Chemistry, vol. 279, no. 4, pp. 2648-2656. https://doi.org/10.1074/jbc.M306849200
Kaduce TL, Fang X, Harmon SD, Oltman CL, Dellsperger KC, Teesch LM et al. 20-Hydroxyeicosatetraenoic Acid (20-HETE) Metabolism in Coronary Endothelial Cells. Journal of Biological Chemistry. 2004 Jan 23;279(4):2648-2656. https://doi.org/10.1074/jbc.M306849200
Kaduce, Terry L. ; Fang, Xiang ; Harmon, Shawn D. ; Oltman, Christine L. ; Dellsperger, Kevin C ; Teesch, Lynn M. ; Gopal, V. Raj ; Falck, J. R. ; Campbell, William B. ; Weintraub, Neal Lee ; Spector, Arthur A. / 20-Hydroxyeicosatetraenoic Acid (20-HETE) Metabolism in Coronary Endothelial Cells. In: Journal of Biological Chemistry. 2004 ; Vol. 279, No. 4. pp. 2648-2656.
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AU - Dellsperger, Kevin C

AU - Teesch, Lynn M.

AU - Gopal, V. Raj

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