A CD44-Like Endothelial Cell Transmembrane Glycoprotein (GP116) Interacts with Extracellular Matrix and Ankyrin

Lilly Y.W. Bourguignon, Vinata B Lokeshwar, J. He, X. Chen, Gerard J. Bourguignon

Research output: Contribution to journalArticle

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Abstract

We used complementary biochemical and immunological techniques to establish that an endothelial cell transmembrane glycoprotein, GP116, is a CD44-like molecule and binds directly both to extracellular matrix components (e.g., hyaluronic acid) and to ankyrin. The specific characteristics of GP116 are as follows: (i) GP116 can be surface labeled with Na125I and contains a wheat germ agglutinin-binding site(s), indicating that it has an extracellular domain; (ii) GP116 displays immunological cross-reactivity with a panel of CD44 antibodies, shares some peptide similarity with CD44, and has a similar 52-kDa precursor molecule, indicating that it is a CD44-like molecule; (iii) GP116 displays specific hyaluronic acid-binding properties, indicating that it is a hyaluronic acid receptor; (iv) GP116 can be phosphorylated by endogenous protein kinase C activated by 12-O-tetradecanoylphorbol-13-acetate and by exogenously added protein kinase C; and (v) GP116 and a 20-kDa tryptic polypeptide fragment of GP116 from the intracellular domain are capable of binding the membrane-cytoskeleton linker molecule, ankyrin. Furthermore, phosphorylation of GP116 by protein kinase C significantly enhances GP116 binding to ankyrin. Together, these findings strongly suggest that phosphorylation of the transmembrane glycoprotein GP116 (a CD44-like molecule) by protein kinase C is required for effective GP116-ankyrin interaction during endothelial cell adhesion events.

Original languageEnglish (US)
Pages (from-to)4464-4471
Number of pages8
JournalMolecular and Cellular Biology
Volume12
Issue number10
DOIs
StatePublished - Jan 1 1992
Externally publishedYes

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Ankyrins
Protein Kinase C
Extracellular Matrix
Glycoproteins
Hyaluronic Acid
Endothelial Cells
Phosphorylation
Immunologic Techniques
Peptides
Wheat Germ Agglutinins
Tetradecanoylphorbol Acetate
Cytoskeleton
Cell Adhesion
Binding Sites
Membranes
Antibodies

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

A CD44-Like Endothelial Cell Transmembrane Glycoprotein (GP116) Interacts with Extracellular Matrix and Ankyrin. / Bourguignon, Lilly Y.W.; Lokeshwar, Vinata B; He, J.; Chen, X.; Bourguignon, Gerard J.

In: Molecular and Cellular Biology, Vol. 12, No. 10, 01.01.1992, p. 4464-4471.

Research output: Contribution to journalArticle

Bourguignon, Lilly Y.W. ; Lokeshwar, Vinata B ; He, J. ; Chen, X. ; Bourguignon, Gerard J. / A CD44-Like Endothelial Cell Transmembrane Glycoprotein (GP116) Interacts with Extracellular Matrix and Ankyrin. In: Molecular and Cellular Biology. 1992 ; Vol. 12, No. 10. pp. 4464-4471.
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