A collagen-based scaffold for a tissue engineered human cornea: Physical and physiological properties

C. J. Doillon, Mitchell A. Watsky, M. Hakim, J. Wang, R. Munger, N. Laycock, R. Osborne, M. Griffith

Research output: Contribution to journalArticle

88 Scopus citations

Abstract

Stabilized collagen-glycosaminoglycan scaffolds for tissue engineered human corneas were characterized. Hydrated matrices were constructed by blending type I collagen with chondroitin sulphates (CS), with glutaraldehyde crosslinking. A corneal keratocyte cell line was added to the scaffolds with or without corneal epithelial and endothelial cells. Constructs were grown with or without ascorbic acid. Wound-healing was evaluated in chemical-treated constructs. Native, noncrosslinked gels were soft with limited longevity. Crosslinking strengthened the matrix yet permitted cell growth. CS addition increased transparency. Keratocytes grown within the matrix had higher frequencies of K+ channel expression than keratocytes grown on plastic. Ascorbic acid increased uncrosslinked matrix degradation in the presence of keratocytes, while it enhanced keratocyte growth and endogenous collagen synthesis in crosslinked matrices. Wounded constructs showed recovery from exposure to chemical irritants. In conclusion, this study demonstrates that our engineered, stabilized matrix is well-suited to function as an in vitro corneal stroma.

Original languageEnglish (US)
Pages (from-to)764-773
Number of pages10
JournalInternational Journal of Artificial Organs
Volume26
Issue number8
Publication statusPublished - Aug 1 2003
Externally publishedYes

    Fingerprint

Keywords

  • Artificial cornea
  • Ascorbic acid
  • Extracellular matrix
  • Keratocyte
  • Tissue engineering
  • Wound healing

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering

Cite this

Doillon, C. J., Watsky, M. A., Hakim, M., Wang, J., Munger, R., Laycock, N., ... Griffith, M. (2003). A collagen-based scaffold for a tissue engineered human cornea: Physical and physiological properties. International Journal of Artificial Organs, 26(8), 764-773.