A common spectrum of polypeptides occurs in secretion granule membranes of different exocrine granules

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Abstract

A highly purified membane preparation from rat parotid secretion has been used as a comparative probe to examine the extent of compositional overlap in granule membranes of three other exocrine secretory tissues - pancreatic, lacrimal, and submandibular - from several standpoints. First, indirect immunofluorescent studies using a polyclonal polyspecific anti-parotid granule membrane antiserum has indicated a selective staining of granule membrane profiles in all acinar cells of all tissues. Second, highly purified granule membrane subfractions have been isolated from each exocrine tissue; comparative two-dimensional (isoelectric focusing; SDS) PAGE of radioiodinated granule membranes has identified 10-15 polypeptides of identical pI and apparent molecular mass. These species are likely to be integral membrane components since they are not extracted by either saponin-sodium or sodium carbonate (pH 11.5) treatments, and they do not have counterparts in the granule content. Finally, the identity among selected parotid and pancreatic radioiodinated granule membrane polypeptides has been documented using two-dimensional peptide mapping of chymotryptic and tryptic digests. These findings clearly indicate that exocrine secretory granules, irrespective of the nature of stored secretion, comprise a type of vesicular carrier with a common (and probably refined) membrane composition. Conceivably, the polypeptides identified carry out general functions related to exocrine secretion.

Original languageEnglish (US)
Pages (from-to)1299-1313
Number of pages15
JournalJournal of Cell Biology
Volume103
Issue number4
DOIs
StatePublished - Jan 1 1986
Externally publishedYes

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Peptides
Membranes
Peptide Mapping
Acinar Cells
Saponins
Secretory Vesicles
Isoelectric Focusing
Tears
Immune Sera
Polyacrylamide Gel Electrophoresis
Sodium
Staining and Labeling

ASJC Scopus subject areas

  • Cell Biology

Cite this

A common spectrum of polypeptides occurs in secretion granule membranes of different exocrine granules. / Cameron, Richard S; Cameron, Patricia L; Castle, J. D.

In: Journal of Cell Biology, Vol. 103, No. 4, 01.01.1986, p. 1299-1313.

Research output: Contribution to journalArticle

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