A comparison of the phenotype of dendritic cells derived from discrete Peyer's patch macrophages of non-infected and Toxoplasma gondii infected mice

Levi H.C. Makala, Julio C.S. Reyes, Yoshifumi Nishikawa, Yoshinori Tsushima, Xuenan Xuan, Xiaohong Huang, Hideyuki Nagasawa

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A comparison of the expression of surface membrane antigens between dendritic cells (DC) derived from Peyer's patch macrophages (DPP-DC) of non-infected and Toxoplasma gondii (T. gondii) infected mice was performed. C57BL/6J mice aged 6-8 weeks of both sexes were infected orally with a 0.5 ml suspension containing 2 × 104 bradyzoites of the Beverley strain of T. gondii, sacrificed on day 8 and DC generated using discrete Peyer's patch macrophages (DPP-Mø) as progenitor cells. When a comparison of the expression of surface membrane antigens between the antigen presenting cells (APC) obtained from discrete Peyer's patches of non-infected and T. gondii infected mice was carried out, no significant differences were observed in the macrophage progenitor and DC populations expression of F4/80, DEC-205, CD11c, CD80 (B7-1) and CD34. However, a significant decrease in MHC class II antigen levels and a down regulation of the co-stimulatory molecule CD86 (B7-2) were noted. B7-1 appeared to be the dominant co-stimulatory ligand, whereas B7-2, which was down regulated during T. gondii infection, had a weak expression. Taken together, these results may help clarify the role of DC in the complex network regulating surface membrane antigens, as well as, their capacity for antigen uptake, processing and presentation during toxoplasmosis.

Original languageEnglish (US)
Pages (from-to)591-597
Number of pages7
JournalJournal of Veterinary Medical Science
Volume65
Issue number5
DOIs
StatePublished - Jan 1 2003

Fingerprint

Peyer's Patches
Peyer's patches
Toxoplasma
dendritic cells
Toxoplasma gondii
Dendritic Cells
macrophages
Macrophages
antigens
Phenotype
phenotype
Surface Antigens
mice
Toxoplasmosis
Antigen Presentation
Membranes
stem cells
Stem Cells
B7 Antigens
bradyzoites

Keywords

  • Dendritic cell
  • Mouse
  • Peyer's patch macrophage
  • Progenitor macrophage
  • Toxoplasma gondii

ASJC Scopus subject areas

  • veterinary(all)

Cite this

A comparison of the phenotype of dendritic cells derived from discrete Peyer's patch macrophages of non-infected and Toxoplasma gondii infected mice. / Makala, Levi H.C.; Reyes, Julio C.S.; Nishikawa, Yoshifumi; Tsushima, Yoshinori; Xuan, Xuenan; Huang, Xiaohong; Nagasawa, Hideyuki.

In: Journal of Veterinary Medical Science, Vol. 65, No. 5, 01.01.2003, p. 591-597.

Research output: Contribution to journalArticle

Makala, Levi H.C. ; Reyes, Julio C.S. ; Nishikawa, Yoshifumi ; Tsushima, Yoshinori ; Xuan, Xuenan ; Huang, Xiaohong ; Nagasawa, Hideyuki. / A comparison of the phenotype of dendritic cells derived from discrete Peyer's patch macrophages of non-infected and Toxoplasma gondii infected mice. In: Journal of Veterinary Medical Science. 2003 ; Vol. 65, No. 5. pp. 591-597.
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