A compendium of developmental gene expression in Lake Malawi cichlid fishes

R. F. Bloomquist, T. E. Fowler, J. B. Sylvester, R. J. Miro, J. T. Streelman

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background: Lake Malawi cichlids represent one of a growing number of vertebrate models used to uncover the genetic and developmental basis of trait diversity. Rapid evolutionary radiation has resulted in species that share similar genomes but differ markedly in phenotypes including brains and behavior, nuptial coloration and the craniofacial skeleton. Research has begun to identify the genes, as well as the molecular and developmental pathways that underlie trait divergence. Results: We assemble a compendium of gene expression for Lake Malawi cichlids, across pharyngula (the phylotypic stage) and larval stages of development, encompassing hundreds of gene transcripts. We chart patterns of expression in Bone morphogenetic protein (BMP), Fibroblast growth factor (FGF), Hedgehog (Hh), Notch and Wingless (Wnt) signaling pathways, as well as genes involved in neurogenesis, calcium and endocrine signaling, stem cell biology, and numerous homeobox (Hox) factors - in three planes using whole-mount in situ hybridization. Because of low sequence divergence across the Malawi cichlid assemblage, the probes we employ are broadly applicable in hundreds of species. We tabulate gene expression across general tissue domains, and highlight examples of unexpected expression patterns. Conclusions: On the heels of recently published genomes, this compendium of developmental gene expression in Lake Malawi cichlids provides a valuable resource for those interested in the relationship between evolution and development.

Original languageEnglish (US)
Article number3
JournalBMC Developmental Biology
Volume17
Issue number1
DOIs
StatePublished - Feb 3 2017

Fingerprint

Developmental Genes
Cichlids
Malawi
Lakes
Gene Expression
Genome
Genes
Bone Morphogenetic Proteins
Wnt Signaling Pathway
Calcium Signaling
Hedgehogs
Fibroblast Growth Factors
Heel
Homeobox Genes
Neurogenesis
Skeleton
In Situ Hybridization
Cell Biology
Vertebrates
Stem Cells

Keywords

  • Cichlid fishes
  • Developmental pathways
  • Evolution of gene expression
  • Lake Malawi

ASJC Scopus subject areas

  • Developmental Biology

Cite this

A compendium of developmental gene expression in Lake Malawi cichlid fishes. / Bloomquist, R. F.; Fowler, T. E.; Sylvester, J. B.; Miro, R. J.; Streelman, J. T.

In: BMC Developmental Biology, Vol. 17, No. 1, 3, 03.02.2017.

Research output: Contribution to journalArticle

Bloomquist, R. F. ; Fowler, T. E. ; Sylvester, J. B. ; Miro, R. J. ; Streelman, J. T. / A compendium of developmental gene expression in Lake Malawi cichlid fishes. In: BMC Developmental Biology. 2017 ; Vol. 17, No. 1.
@article{6c2e477d351947c5a632bdeea88b67e2,
title = "A compendium of developmental gene expression in Lake Malawi cichlid fishes",
abstract = "Background: Lake Malawi cichlids represent one of a growing number of vertebrate models used to uncover the genetic and developmental basis of trait diversity. Rapid evolutionary radiation has resulted in species that share similar genomes but differ markedly in phenotypes including brains and behavior, nuptial coloration and the craniofacial skeleton. Research has begun to identify the genes, as well as the molecular and developmental pathways that underlie trait divergence. Results: We assemble a compendium of gene expression for Lake Malawi cichlids, across pharyngula (the phylotypic stage) and larval stages of development, encompassing hundreds of gene transcripts. We chart patterns of expression in Bone morphogenetic protein (BMP), Fibroblast growth factor (FGF), Hedgehog (Hh), Notch and Wingless (Wnt) signaling pathways, as well as genes involved in neurogenesis, calcium and endocrine signaling, stem cell biology, and numerous homeobox (Hox) factors - in three planes using whole-mount in situ hybridization. Because of low sequence divergence across the Malawi cichlid assemblage, the probes we employ are broadly applicable in hundreds of species. We tabulate gene expression across general tissue domains, and highlight examples of unexpected expression patterns. Conclusions: On the heels of recently published genomes, this compendium of developmental gene expression in Lake Malawi cichlids provides a valuable resource for those interested in the relationship between evolution and development.",
keywords = "Cichlid fishes, Developmental pathways, Evolution of gene expression, Lake Malawi",
author = "Bloomquist, {R. F.} and Fowler, {T. E.} and Sylvester, {J. B.} and Miro, {R. J.} and Streelman, {J. T.}",
year = "2017",
month = "2",
day = "3",
doi = "10.1186/s12861-017-0146-0",
language = "English (US)",
volume = "17",
journal = "BMC Developmental Biology",
issn = "1471-213X",
publisher = "BioMed Central",
number = "1",

}

TY - JOUR

T1 - A compendium of developmental gene expression in Lake Malawi cichlid fishes

AU - Bloomquist, R. F.

AU - Fowler, T. E.

AU - Sylvester, J. B.

AU - Miro, R. J.

AU - Streelman, J. T.

PY - 2017/2/3

Y1 - 2017/2/3

N2 - Background: Lake Malawi cichlids represent one of a growing number of vertebrate models used to uncover the genetic and developmental basis of trait diversity. Rapid evolutionary radiation has resulted in species that share similar genomes but differ markedly in phenotypes including brains and behavior, nuptial coloration and the craniofacial skeleton. Research has begun to identify the genes, as well as the molecular and developmental pathways that underlie trait divergence. Results: We assemble a compendium of gene expression for Lake Malawi cichlids, across pharyngula (the phylotypic stage) and larval stages of development, encompassing hundreds of gene transcripts. We chart patterns of expression in Bone morphogenetic protein (BMP), Fibroblast growth factor (FGF), Hedgehog (Hh), Notch and Wingless (Wnt) signaling pathways, as well as genes involved in neurogenesis, calcium and endocrine signaling, stem cell biology, and numerous homeobox (Hox) factors - in three planes using whole-mount in situ hybridization. Because of low sequence divergence across the Malawi cichlid assemblage, the probes we employ are broadly applicable in hundreds of species. We tabulate gene expression across general tissue domains, and highlight examples of unexpected expression patterns. Conclusions: On the heels of recently published genomes, this compendium of developmental gene expression in Lake Malawi cichlids provides a valuable resource for those interested in the relationship between evolution and development.

AB - Background: Lake Malawi cichlids represent one of a growing number of vertebrate models used to uncover the genetic and developmental basis of trait diversity. Rapid evolutionary radiation has resulted in species that share similar genomes but differ markedly in phenotypes including brains and behavior, nuptial coloration and the craniofacial skeleton. Research has begun to identify the genes, as well as the molecular and developmental pathways that underlie trait divergence. Results: We assemble a compendium of gene expression for Lake Malawi cichlids, across pharyngula (the phylotypic stage) and larval stages of development, encompassing hundreds of gene transcripts. We chart patterns of expression in Bone morphogenetic protein (BMP), Fibroblast growth factor (FGF), Hedgehog (Hh), Notch and Wingless (Wnt) signaling pathways, as well as genes involved in neurogenesis, calcium and endocrine signaling, stem cell biology, and numerous homeobox (Hox) factors - in three planes using whole-mount in situ hybridization. Because of low sequence divergence across the Malawi cichlid assemblage, the probes we employ are broadly applicable in hundreds of species. We tabulate gene expression across general tissue domains, and highlight examples of unexpected expression patterns. Conclusions: On the heels of recently published genomes, this compendium of developmental gene expression in Lake Malawi cichlids provides a valuable resource for those interested in the relationship between evolution and development.

KW - Cichlid fishes

KW - Developmental pathways

KW - Evolution of gene expression

KW - Lake Malawi

UR - http://www.scopus.com/inward/record.url?scp=85011395634&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85011395634&partnerID=8YFLogxK

U2 - 10.1186/s12861-017-0146-0

DO - 10.1186/s12861-017-0146-0

M3 - Article

C2 - 28158974

AN - SCOPUS:85011395634

VL - 17

JO - BMC Developmental Biology

JF - BMC Developmental Biology

SN - 1471-213X

IS - 1

M1 - 3

ER -