A glycophospholipid anchor is required for Qa-2-mediated T cell activation

P. J. Robinson, M. Millrain, J. Antoniou, E. Simpson, A. L. Mellor

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Abstract

A NUMBER of lymphocyte surface proteins are anchored in the cell membrane by glycophosphatidyl inositol (known as GPI) linkages instead of hydrophobic protein domains1,2. Treatment of mouse T lymphocytes with antibodies specific for two such proteins, Thy-1 and Ly-6, are known to induce proliferation3,4. We have found that antibodies specific for Qa-2, a GPI-anchored class I histocompatibility antigen5, can also activate mouse T cells. To determine whether the GPI-anchor is important for this pathway of cell activation, we produced transgenic mice expressing either normal GPI-anchored Qa-2, or Qa-2 molecules with a membrane-spanning protein domain derived from H-2. Our studies show that only lymphocytes from transgenic mice carrying GPI-anchored forms of Qa-2 can be activated in vitro by Qa-2-specific antibodies. We also show that transgenic mouse T cells expressing a GPI-anchored form of H-2Db can be activated by anti-H-2Db antibodies. These results strongly indicate that the GPI-anchor is critical for this pathway of T cell activation.

Original languageEnglish (US)
Pages (from-to)85-87
Number of pages3
JournalNature
Volume342
Issue number6245
DOIs
StatePublished - Jan 1 1989

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    Robinson, P. J., Millrain, M., Antoniou, J., Simpson, E., & Mellor, A. L. (1989). A glycophospholipid anchor is required for Qa-2-mediated T cell activation. Nature, 342(6245), 85-87. https://doi.org/10.1038/342085a0