A leptin dose-response study in obese (ob/ob) and lean (+/?) mice

Ruth Babette Harris, Jun Zhou, Stephen M. Redmann, Gennady N. Smagin, Steven R. Smith, Erin Rodgers, Jeffrey J. Zachwieja

Research output: Contribution to journalArticle

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Abstract

This experiment determined the amount of leptin required to correct different abnormalities in leptin-deficient ob/ob mice. Baseline food intakes and body weights of lean (+/?) and obese (ob/ob) C57Bl/6J <ob= mice were recorded for 7 days. An Alzet miniosmotic pump was placed in the peritoneal cavity of each mouse and delivered 0, 1, 2, 5, 10, or 42 μg/day human leptin for 7 days. In ob/ob mice, 2 μg leptin/day reduced food intake and body weight, and increased hypothalamic and brain stem serotonin concentrations. All fat pads were reduced 35-40% by 10 μg leptin/day, and liver weight, lipid, and glycogen decreased. Serum insulin and glucose were reduced in all leptin-treated ob/ob mice, and levels were normalized by 10 μg/day leptin. Low rectal temperatures of ob/ob mice were corrected by 10 and 42 μg/day leptin. These doses also increased brown adipose tissue uncoupling protein expression. The only responses in lean mice were a transient reduction in food intake and weight loss with 10 or 42 μg/day leptin. This study shows enhanced leptin sensitivity in ob/ob mice and suggests that increased temperature and sympathetic activity are indirect responses to high concentrations of protein.

Original languageEnglish (US)
Pages (from-to)8-19
Number of pages12
JournalEndocrinology
Volume139
Issue number1
DOIs
StatePublished - Jan 1 1998
Externally publishedYes

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Leptin
Eating
Body Weight
Temperature
Peritoneal Cavity
Glycogen
Brain Stem
Adipose Tissue
Weight Loss
Serotonin
Insulin
Lipids
Weights and Measures
Glucose
Liver

ASJC Scopus subject areas

  • Endocrinology

Cite this

Harris, R. B., Zhou, J., Redmann, S. M., Smagin, G. N., Smith, S. R., Rodgers, E., & Zachwieja, J. J. (1998). A leptin dose-response study in obese (ob/ob) and lean (+/?) mice. Endocrinology, 139(1), 8-19. https://doi.org/10.1210/endo.139.1.5675

A leptin dose-response study in obese (ob/ob) and lean (+/?) mice. / Harris, Ruth Babette; Zhou, Jun; Redmann, Stephen M.; Smagin, Gennady N.; Smith, Steven R.; Rodgers, Erin; Zachwieja, Jeffrey J.

In: Endocrinology, Vol. 139, No. 1, 01.01.1998, p. 8-19.

Research output: Contribution to journalArticle

Harris, RB, Zhou, J, Redmann, SM, Smagin, GN, Smith, SR, Rodgers, E & Zachwieja, JJ 1998, 'A leptin dose-response study in obese (ob/ob) and lean (+/?) mice', Endocrinology, vol. 139, no. 1, pp. 8-19. https://doi.org/10.1210/endo.139.1.5675
Harris RB, Zhou J, Redmann SM, Smagin GN, Smith SR, Rodgers E et al. A leptin dose-response study in obese (ob/ob) and lean (+/?) mice. Endocrinology. 1998 Jan 1;139(1):8-19. https://doi.org/10.1210/endo.139.1.5675
Harris, Ruth Babette ; Zhou, Jun ; Redmann, Stephen M. ; Smagin, Gennady N. ; Smith, Steven R. ; Rodgers, Erin ; Zachwieja, Jeffrey J. / A leptin dose-response study in obese (ob/ob) and lean (+/?) mice. In: Endocrinology. 1998 ; Vol. 139, No. 1. pp. 8-19.
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