A liver specific gene that is expressed in growth hormone transgenic mice and in normal female mice as a function of age

Jean D.R. Tiong, Elahu Gosney, Juan Ding, Edward Chin, John J. Kopchick

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Growth hormone (GH) acts on various organs to exert its growth and metabolic effects. GH induces transcription of a number of genes in different organs including liver. By performing subtractive hybridization analysis on liver cDNAs of GH transgenic and non-transgenic mice, differentially expressed cDNAs were obtained. This paper describes the isolation and characterization of a liver cDNA, termed cDNA #5, that contains 1897 bp and is predicted to encode a protein (P5) of 512 aa residues. P5 has five immunoglobulin related domains thus allowing it to be classified as a member of the immunoglobulin super family (IGSF). Also, P5 shows significant similarity to both rat and human alpha-1-B glycoprotein which is an acidic serum protein of unknown function. mRNA #5 was detected in the liver hepatocytes of male and female GH transgenic mice and in the liver of female, but not of male, non-transgenic mice. mRNA #5 was not present in dwarf mice including the Ames dwarf, those that express a GH antagonist and those with the GH receptor and binding protein gene disrupted. These findings suggest that induction of mRNA #5 in the liver requires a continuous pattern of GH secretion and an intact GH-GH receptor-signaling complex. mRNA #5 levels in female non-transgenic mice were observed to vary with age implying that gender-specific age-dependent factor(s) may be involved in the induction of mRNA #5. The appearance of mRNA #5 in post-hepatectomized liver that coincides with the proliferative phase of liver regeneration suggests that it may be involved in hepatocyte proliferation. Together these data suggest that expression of cDNA #5 is liver-specific, sexually dimorphic, age-dependent, and may be involved in hepatocyte hyperplasia and liver enlargement.

Original languageEnglish (US)
Pages (from-to)145-156
Number of pages12
JournalGrowth Hormone and IGF Research
Volume16
Issue number3
DOIs
StatePublished - Jun 1 2006

Fingerprint

Transgenic Mice
Growth Hormone
Liver
Genes
Complementary DNA
Messenger RNA
Somatotropin Receptors
Hepatocytes
Hormone Antagonists
Liver Regeneration
Age Factors
Hyperplasia
Immunoglobulins
Blood Proteins
Glycoproteins
Growth

Keywords

  • Aging
  • Alpha-1B-glycoprotein
  • GH
  • GH transgenic mice
  • GH-regulated genes
  • Immunoglobulin super family
  • Liver regeneration
  • Sexual dimorphism
  • Subtractive cDNA hybridization

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Cite this

A liver specific gene that is expressed in growth hormone transgenic mice and in normal female mice as a function of age. / Tiong, Jean D.R.; Gosney, Elahu; Ding, Juan; Chin, Edward; Kopchick, John J.

In: Growth Hormone and IGF Research, Vol. 16, No. 3, 01.06.2006, p. 145-156.

Research output: Contribution to journalArticle

Tiong, Jean D.R. ; Gosney, Elahu ; Ding, Juan ; Chin, Edward ; Kopchick, John J. / A liver specific gene that is expressed in growth hormone transgenic mice and in normal female mice as a function of age. In: Growth Hormone and IGF Research. 2006 ; Vol. 16, No. 3. pp. 145-156.
@article{450e76f188704cdca1a7e4ef12ff4205,
title = "A liver specific gene that is expressed in growth hormone transgenic mice and in normal female mice as a function of age",
abstract = "Growth hormone (GH) acts on various organs to exert its growth and metabolic effects. GH induces transcription of a number of genes in different organs including liver. By performing subtractive hybridization analysis on liver cDNAs of GH transgenic and non-transgenic mice, differentially expressed cDNAs were obtained. This paper describes the isolation and characterization of a liver cDNA, termed cDNA #5, that contains 1897 bp and is predicted to encode a protein (P5) of 512 aa residues. P5 has five immunoglobulin related domains thus allowing it to be classified as a member of the immunoglobulin super family (IGSF). Also, P5 shows significant similarity to both rat and human alpha-1-B glycoprotein which is an acidic serum protein of unknown function. mRNA #5 was detected in the liver hepatocytes of male and female GH transgenic mice and in the liver of female, but not of male, non-transgenic mice. mRNA #5 was not present in dwarf mice including the Ames dwarf, those that express a GH antagonist and those with the GH receptor and binding protein gene disrupted. These findings suggest that induction of mRNA #5 in the liver requires a continuous pattern of GH secretion and an intact GH-GH receptor-signaling complex. mRNA #5 levels in female non-transgenic mice were observed to vary with age implying that gender-specific age-dependent factor(s) may be involved in the induction of mRNA #5. The appearance of mRNA #5 in post-hepatectomized liver that coincides with the proliferative phase of liver regeneration suggests that it may be involved in hepatocyte proliferation. Together these data suggest that expression of cDNA #5 is liver-specific, sexually dimorphic, age-dependent, and may be involved in hepatocyte hyperplasia and liver enlargement.",
keywords = "Aging, Alpha-1B-glycoprotein, GH, GH transgenic mice, GH-regulated genes, Immunoglobulin super family, Liver regeneration, Sexual dimorphism, Subtractive cDNA hybridization",
author = "Tiong, {Jean D.R.} and Elahu Gosney and Juan Ding and Edward Chin and Kopchick, {John J.}",
year = "2006",
month = "6",
day = "1",
doi = "10.1016/j.ghir.2006.03.011",
language = "English (US)",
volume = "16",
pages = "145--156",
journal = "Growth Hormone and IGF Research",
issn = "1096-6374",
publisher = "Elsevier Ltd",
number = "3",

}

TY - JOUR

T1 - A liver specific gene that is expressed in growth hormone transgenic mice and in normal female mice as a function of age

AU - Tiong, Jean D.R.

AU - Gosney, Elahu

AU - Ding, Juan

AU - Chin, Edward

AU - Kopchick, John J.

PY - 2006/6/1

Y1 - 2006/6/1

N2 - Growth hormone (GH) acts on various organs to exert its growth and metabolic effects. GH induces transcription of a number of genes in different organs including liver. By performing subtractive hybridization analysis on liver cDNAs of GH transgenic and non-transgenic mice, differentially expressed cDNAs were obtained. This paper describes the isolation and characterization of a liver cDNA, termed cDNA #5, that contains 1897 bp and is predicted to encode a protein (P5) of 512 aa residues. P5 has five immunoglobulin related domains thus allowing it to be classified as a member of the immunoglobulin super family (IGSF). Also, P5 shows significant similarity to both rat and human alpha-1-B glycoprotein which is an acidic serum protein of unknown function. mRNA #5 was detected in the liver hepatocytes of male and female GH transgenic mice and in the liver of female, but not of male, non-transgenic mice. mRNA #5 was not present in dwarf mice including the Ames dwarf, those that express a GH antagonist and those with the GH receptor and binding protein gene disrupted. These findings suggest that induction of mRNA #5 in the liver requires a continuous pattern of GH secretion and an intact GH-GH receptor-signaling complex. mRNA #5 levels in female non-transgenic mice were observed to vary with age implying that gender-specific age-dependent factor(s) may be involved in the induction of mRNA #5. The appearance of mRNA #5 in post-hepatectomized liver that coincides with the proliferative phase of liver regeneration suggests that it may be involved in hepatocyte proliferation. Together these data suggest that expression of cDNA #5 is liver-specific, sexually dimorphic, age-dependent, and may be involved in hepatocyte hyperplasia and liver enlargement.

AB - Growth hormone (GH) acts on various organs to exert its growth and metabolic effects. GH induces transcription of a number of genes in different organs including liver. By performing subtractive hybridization analysis on liver cDNAs of GH transgenic and non-transgenic mice, differentially expressed cDNAs were obtained. This paper describes the isolation and characterization of a liver cDNA, termed cDNA #5, that contains 1897 bp and is predicted to encode a protein (P5) of 512 aa residues. P5 has five immunoglobulin related domains thus allowing it to be classified as a member of the immunoglobulin super family (IGSF). Also, P5 shows significant similarity to both rat and human alpha-1-B glycoprotein which is an acidic serum protein of unknown function. mRNA #5 was detected in the liver hepatocytes of male and female GH transgenic mice and in the liver of female, but not of male, non-transgenic mice. mRNA #5 was not present in dwarf mice including the Ames dwarf, those that express a GH antagonist and those with the GH receptor and binding protein gene disrupted. These findings suggest that induction of mRNA #5 in the liver requires a continuous pattern of GH secretion and an intact GH-GH receptor-signaling complex. mRNA #5 levels in female non-transgenic mice were observed to vary with age implying that gender-specific age-dependent factor(s) may be involved in the induction of mRNA #5. The appearance of mRNA #5 in post-hepatectomized liver that coincides with the proliferative phase of liver regeneration suggests that it may be involved in hepatocyte proliferation. Together these data suggest that expression of cDNA #5 is liver-specific, sexually dimorphic, age-dependent, and may be involved in hepatocyte hyperplasia and liver enlargement.

KW - Aging

KW - Alpha-1B-glycoprotein

KW - GH

KW - GH transgenic mice

KW - GH-regulated genes

KW - Immunoglobulin super family

KW - Liver regeneration

KW - Sexual dimorphism

KW - Subtractive cDNA hybridization

UR - http://www.scopus.com/inward/record.url?scp=33746291097&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33746291097&partnerID=8YFLogxK

U2 - 10.1016/j.ghir.2006.03.011

DO - 10.1016/j.ghir.2006.03.011

M3 - Article

VL - 16

SP - 145

EP - 156

JO - Growth Hormone and IGF Research

JF - Growth Hormone and IGF Research

SN - 1096-6374

IS - 3

ER -