A liver specific gene that is expressed in growth hormone transgenic mice and in normal female mice as a function of age

Jean D.R. Tiong, Elahu Gosney, Juan Ding, Edward Chin, John J. Kopchick

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Growth hormone (GH) acts on various organs to exert its growth and metabolic effects. GH induces transcription of a number of genes in different organs including liver. By performing subtractive hybridization analysis on liver cDNAs of GH transgenic and non-transgenic mice, differentially expressed cDNAs were obtained. This paper describes the isolation and characterization of a liver cDNA, termed cDNA #5, that contains 1897 bp and is predicted to encode a protein (P5) of 512 aa residues. P5 has five immunoglobulin related domains thus allowing it to be classified as a member of the immunoglobulin super family (IGSF). Also, P5 shows significant similarity to both rat and human alpha-1-B glycoprotein which is an acidic serum protein of unknown function. mRNA #5 was detected in the liver hepatocytes of male and female GH transgenic mice and in the liver of female, but not of male, non-transgenic mice. mRNA #5 was not present in dwarf mice including the Ames dwarf, those that express a GH antagonist and those with the GH receptor and binding protein gene disrupted. These findings suggest that induction of mRNA #5 in the liver requires a continuous pattern of GH secretion and an intact GH-GH receptor-signaling complex. mRNA #5 levels in female non-transgenic mice were observed to vary with age implying that gender-specific age-dependent factor(s) may be involved in the induction of mRNA #5. The appearance of mRNA #5 in post-hepatectomized liver that coincides with the proliferative phase of liver regeneration suggests that it may be involved in hepatocyte proliferation. Together these data suggest that expression of cDNA #5 is liver-specific, sexually dimorphic, age-dependent, and may be involved in hepatocyte hyperplasia and liver enlargement.

Original languageEnglish (US)
Pages (from-to)145-156
Number of pages12
JournalGrowth Hormone and IGF Research
Volume16
Issue number3
DOIs
StatePublished - Jun 1 2006
Externally publishedYes

Keywords

  • Aging
  • Alpha-1B-glycoprotein
  • GH
  • GH transgenic mice
  • GH-regulated genes
  • Immunoglobulin super family
  • Liver regeneration
  • Sexual dimorphism
  • Subtractive cDNA hybridization

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Fingerprint Dive into the research topics of 'A liver specific gene that is expressed in growth hormone transgenic mice and in normal female mice as a function of age'. Together they form a unique fingerprint.

  • Cite this