A mechanism-based in vitro anticancer drug screening approach for phenolic phytochemicals.

Stephen Hsu, Fu Xin X. Yu, Qin Huang, Jill Lewis, Baldev Singh, Douglas Dickinson, James Borke, Mohamed M.H. Sharawy, John Wataha, Tetsuya Yamamoto, Tokio Osaki, George Schuster

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Plant-derived phenolic compounds, including polyphenols (e.g., tannins), flavonoids, and phenolic acids, have been under investigation for their anticancer therapeutic and chemoprevention properties. Recently, certain mechanisms underlying the differential effects of green tea polyphenols (GTPPs) on tumor versus normal cells have been determined. These suggest that GTPPs may simultaneously activate multiple pathways. However, existing screening methods are insufficient for the identification of agents that possess both a cytotoxic effect on tumor cells and a protective effect on normal cells. The current study describes the establishment of an in vitro survival/apoptosis testing system based on detecting these mechanisms by a double-fluorescence method. This system is able to screen potential chemopreventive or therapeutic agents from (but not limited to) plant-derived compounds based on the pathways differentially activated by the agents. Tumor cell death and normal cell survival are detected simultaneously, in a device that co-cultures normal human cells adjacent to human tumor cells.

Original languageEnglish (US)
Pages (from-to)611-618
Number of pages8
JournalAssay and Drug Development Technologies
Volume1
Issue number5
DOIs
StatePublished - Jan 1 2003

ASJC Scopus subject areas

  • Molecular Medicine
  • Drug Discovery

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    Hsu, S., Yu, F. X. X., Huang, Q., Lewis, J., Singh, B., Dickinson, D., Borke, J., Sharawy, M. M. H., Wataha, J., Yamamoto, T., Osaki, T., & Schuster, G. (2003). A mechanism-based in vitro anticancer drug screening approach for phenolic phytochemicals. Assay and Drug Development Technologies, 1(5), 611-618. https://doi.org/10.1089/154065803770380968