A method for the in vitro determination of feline corneal endothelial permeability

Mark L. Mcdermott, Mitchell A. Watsky, Dayle H. Geroski, Henry F. Edelhauser

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

A mounting block for in vitro perfusion of the cat cornea is described. Using this apparatus and the techniques of Araie, the permeability (Pac) of the normal cat corneal endothelium to carboxyfluorscein was determined to be 2.5 plusmn; 0.2 × 10-4 cm/min. To assess the sensitivity of this technique in determining changes in Pac associated with alterations in endothelial, morphology, three cats underwent 2 successive unilateral, central, 10 mm diameter circular areas of endothelial debridement 6 weeks apart. Six weeks following the second wounding all 3 animals underwent morphometric analysis and Pac determination. A trend toward an elevation in Pac with extreme reductions in cell density was observed.

Original languageEnglish (US)
Pages (from-to)1129-1136
Number of pages8
JournalCurrent Eye Research
Volume9
Issue number12
DOIs
StatePublished - Jan 1 1990
Externally publishedYes

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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