A model of lysosomal metabolism of dextran coated superparamagnetic iron oxide (SPIO) nanoparticles: Implications for cellular magnetic resonance imaging

Ali Syed Arbab, Lindsey B. Wilson, Parwana Ashari, Elaine K. Jordan, Bobbi K. Lewis, Joseph A. Frank

Research output: Contribution to journalArticle

259 Citations (Scopus)

Abstract

Ferumoxides, dextran-coated superparamagnetic iron oxide (SPIO) particles, form ferumoxide-transfection agent (FE-TA) complexes that are internalized into endosomes/lysosomes and have been used to label cells for in vivo MRI tracking and localization studies. A better understanding of the physical state of the FE-TA complexes during endocytosis could improve their use. The purpose of this study was to measure the rate of the degradation of iron particles under varying physiological conditions. FE-TA complexes were incubated in seven different buffers containing different chelates with different pH. Reducible iron concentrations, T2 relaxation rates and gradient echo (GRE) magnetic resonance images (MRI) were obtained from each condition immediately after incubation and at 6, 24, 48, 72 and 96 h and days 7, 14 and 21. The dynamics of FE-TA in the endosome/lysomes within the cells were visualized with electron microscopy. Sodium citrate buffer at pH 4.5 rapidly dissolved FE-TA complexes. However, FE-TA complexes were less soluble in the same buffer at pH 5.5. Similarly, FE-TA complexes were not readily soluble in any of the other buffers with or without chelates, regardless of pH. Electron microscopic images showed degraded FE-TA in some intracellular endosome/lysosomes between days 3 and 5. In the cellular environment, some of the FE-TA-containing endosomes were found to fuse with lysosomes, causing rapid dissociation at low pH and exposing the iron core to chelates that resulted in soluble Fe(III) within the lysosomes. The studies presented represent a first step in identifying the important cellular environmental parameters affecting the integrity of FE-TA complexes.

Original languageEnglish (US)
Pages (from-to)383-389
Number of pages7
JournalNMR in Biomedicine
Volume18
Issue number6
DOIs
StatePublished - Oct 1 2005

Fingerprint

Magnetic resonance
Metabolism
Nanoparticles
Transfection
Magnetic Resonance Imaging
Imaging techniques
Endosomes
Lysosomes
Buffers
Iron
ferumoxides
Magnetic Resonance Spectroscopy
Electric fuses
Endocytosis
Electron microscopy
Labels
Electron Microscopy
Electrons
Degradation

Keywords

  • Endosome/lysosome
  • Ferumoxides
  • Labeled cells
  • Poly-L-lysine
  • SPIO

ASJC Scopus subject areas

  • Molecular Medicine
  • Radiology Nuclear Medicine and imaging
  • Spectroscopy

Cite this

A model of lysosomal metabolism of dextran coated superparamagnetic iron oxide (SPIO) nanoparticles : Implications for cellular magnetic resonance imaging. / Arbab, Ali Syed; Wilson, Lindsey B.; Ashari, Parwana; Jordan, Elaine K.; Lewis, Bobbi K.; Frank, Joseph A.

In: NMR in Biomedicine, Vol. 18, No. 6, 01.10.2005, p. 383-389.

Research output: Contribution to journalArticle

Arbab, Ali Syed ; Wilson, Lindsey B. ; Ashari, Parwana ; Jordan, Elaine K. ; Lewis, Bobbi K. ; Frank, Joseph A. / A model of lysosomal metabolism of dextran coated superparamagnetic iron oxide (SPIO) nanoparticles : Implications for cellular magnetic resonance imaging. In: NMR in Biomedicine. 2005 ; Vol. 18, No. 6. pp. 383-389.
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