A new approach to ELISA-based anti-glycolipid antibody evaluation of highly adhesive serum samples

Seigo Usuki, Dawn O'Brien, Michael H. Rivner, Robert K. Yu

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The enzyme-linked immunosorbent assay (ELISA) is a standard immunoassay used in measuring antibody reactivity (expressed as titers) for glycosphingolipids (GSLs) such as gangliosides and sulfoglycolipids in the sera of patients with Guillain-Barré syndrome (GBS), variants of GBS, and chronic inflammatory demyelinating polyneuropathy (CIDP). In the present study, anti-GSL antibodies were evaluated using a new formula of affinity parametric complex (APC), calculated from limiting-dilution serum assay data, followed by affinity parametric complex criterion (APCC). Using assay results based on APCC, we analyzed serum samples categorized into acute inflammatory demyelinating polyneuropathy (AIDP), acute motor-sensory axonal neuropathy (AMSAN), CIDP, CIDP with myasthenia gravis (MG), and Amyotrophic Lateral Sclerosis (ALS). We were able to determine the affinity strength of antibodies otherwise hidden in the non-specific background activity in highly adhesive serum samples. The thin-layer chromatography (TLC)-immuno-overlay method assured us that this new method is an accurate and reliable way for evaluating anti-GSL antibodies using ELISA serum sample data.

Original languageEnglish (US)
Pages (from-to)52-63
Number of pages12
JournalJournal of Immunological Methods
Volume408
DOIs
StatePublished - Jun 2014

Fingerprint

Glycolipids
Adhesives
Anti-Idiotypic Antibodies
Chronic Inflammatory Demyelinating Polyradiculoneuropathy
Glycosphingolipids
Enzyme-Linked Immunosorbent Assay
Serum
Guillain-Barre Syndrome
Antibody Affinity
Polyneuropathies
Gangliosides
Myasthenia Gravis
Amyotrophic Lateral Sclerosis
Thin Layer Chromatography
Immunoassay
Antibodies

Keywords

  • ALS
  • Anti-glycolipid antibody
  • CIDP
  • ELISA
  • GBS
  • Non-specific adhesions

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

A new approach to ELISA-based anti-glycolipid antibody evaluation of highly adhesive serum samples. / Usuki, Seigo; O'Brien, Dawn; Rivner, Michael H.; Yu, Robert K.

In: Journal of Immunological Methods, Vol. 408, 06.2014, p. 52-63.

Research output: Contribution to journalArticle

@article{14a0778a614f4e0d89cbe7e598586a9b,
title = "A new approach to ELISA-based anti-glycolipid antibody evaluation of highly adhesive serum samples",
abstract = "The enzyme-linked immunosorbent assay (ELISA) is a standard immunoassay used in measuring antibody reactivity (expressed as titers) for glycosphingolipids (GSLs) such as gangliosides and sulfoglycolipids in the sera of patients with Guillain-Barr{\'e} syndrome (GBS), variants of GBS, and chronic inflammatory demyelinating polyneuropathy (CIDP). In the present study, anti-GSL antibodies were evaluated using a new formula of affinity parametric complex (APC), calculated from limiting-dilution serum assay data, followed by affinity parametric complex criterion (APCC). Using assay results based on APCC, we analyzed serum samples categorized into acute inflammatory demyelinating polyneuropathy (AIDP), acute motor-sensory axonal neuropathy (AMSAN), CIDP, CIDP with myasthenia gravis (MG), and Amyotrophic Lateral Sclerosis (ALS). We were able to determine the affinity strength of antibodies otherwise hidden in the non-specific background activity in highly adhesive serum samples. The thin-layer chromatography (TLC)-immuno-overlay method assured us that this new method is an accurate and reliable way for evaluating anti-GSL antibodies using ELISA serum sample data.",
keywords = "ALS, Anti-glycolipid antibody, CIDP, ELISA, GBS, Non-specific adhesions",
author = "Seigo Usuki and Dawn O'Brien and Rivner, {Michael H.} and Yu, {Robert K.}",
year = "2014",
month = "6",
doi = "10.1016/j.jim.2014.05.005",
language = "English (US)",
volume = "408",
pages = "52--63",
journal = "Journal of Immunological Methods",
issn = "0022-1759",
publisher = "Elsevier",

}

TY - JOUR

T1 - A new approach to ELISA-based anti-glycolipid antibody evaluation of highly adhesive serum samples

AU - Usuki, Seigo

AU - O'Brien, Dawn

AU - Rivner, Michael H.

AU - Yu, Robert K.

PY - 2014/6

Y1 - 2014/6

N2 - The enzyme-linked immunosorbent assay (ELISA) is a standard immunoassay used in measuring antibody reactivity (expressed as titers) for glycosphingolipids (GSLs) such as gangliosides and sulfoglycolipids in the sera of patients with Guillain-Barré syndrome (GBS), variants of GBS, and chronic inflammatory demyelinating polyneuropathy (CIDP). In the present study, anti-GSL antibodies were evaluated using a new formula of affinity parametric complex (APC), calculated from limiting-dilution serum assay data, followed by affinity parametric complex criterion (APCC). Using assay results based on APCC, we analyzed serum samples categorized into acute inflammatory demyelinating polyneuropathy (AIDP), acute motor-sensory axonal neuropathy (AMSAN), CIDP, CIDP with myasthenia gravis (MG), and Amyotrophic Lateral Sclerosis (ALS). We were able to determine the affinity strength of antibodies otherwise hidden in the non-specific background activity in highly adhesive serum samples. The thin-layer chromatography (TLC)-immuno-overlay method assured us that this new method is an accurate and reliable way for evaluating anti-GSL antibodies using ELISA serum sample data.

AB - The enzyme-linked immunosorbent assay (ELISA) is a standard immunoassay used in measuring antibody reactivity (expressed as titers) for glycosphingolipids (GSLs) such as gangliosides and sulfoglycolipids in the sera of patients with Guillain-Barré syndrome (GBS), variants of GBS, and chronic inflammatory demyelinating polyneuropathy (CIDP). In the present study, anti-GSL antibodies were evaluated using a new formula of affinity parametric complex (APC), calculated from limiting-dilution serum assay data, followed by affinity parametric complex criterion (APCC). Using assay results based on APCC, we analyzed serum samples categorized into acute inflammatory demyelinating polyneuropathy (AIDP), acute motor-sensory axonal neuropathy (AMSAN), CIDP, CIDP with myasthenia gravis (MG), and Amyotrophic Lateral Sclerosis (ALS). We were able to determine the affinity strength of antibodies otherwise hidden in the non-specific background activity in highly adhesive serum samples. The thin-layer chromatography (TLC)-immuno-overlay method assured us that this new method is an accurate and reliable way for evaluating anti-GSL antibodies using ELISA serum sample data.

KW - ALS

KW - Anti-glycolipid antibody

KW - CIDP

KW - ELISA

KW - GBS

KW - Non-specific adhesions

UR - http://www.scopus.com/inward/record.url?scp=84903468987&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84903468987&partnerID=8YFLogxK

U2 - 10.1016/j.jim.2014.05.005

DO - 10.1016/j.jim.2014.05.005

M3 - Article

C2 - 24861939

AN - SCOPUS:84903468987

VL - 408

SP - 52

EP - 63

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

SN - 0022-1759

ER -