A new approach to ELISA-based anti-glycolipid antibody evaluation of highly adhesive serum samples

Seigo Usuki, Dawn O'Brien, Michael H. Rivner, Robert K. Yu

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

The enzyme-linked immunosorbent assay (ELISA) is a standard immunoassay used in measuring antibody reactivity (expressed as titers) for glycosphingolipids (GSLs) such as gangliosides and sulfoglycolipids in the sera of patients with Guillain-Barré syndrome (GBS), variants of GBS, and chronic inflammatory demyelinating polyneuropathy (CIDP). In the present study, anti-GSL antibodies were evaluated using a new formula of affinity parametric complex (APC), calculated from limiting-dilution serum assay data, followed by affinity parametric complex criterion (APCC). Using assay results based on APCC, we analyzed serum samples categorized into acute inflammatory demyelinating polyneuropathy (AIDP), acute motor-sensory axonal neuropathy (AMSAN), CIDP, CIDP with myasthenia gravis (MG), and Amyotrophic Lateral Sclerosis (ALS). We were able to determine the affinity strength of antibodies otherwise hidden in the non-specific background activity in highly adhesive serum samples. The thin-layer chromatography (TLC)-immuno-overlay method assured us that this new method is an accurate and reliable way for evaluating anti-GSL antibodies using ELISA serum sample data.

Original languageEnglish (US)
Pages (from-to)52-63
Number of pages12
JournalJournal of Immunological Methods
Volume408
DOIs
StatePublished - Jun 2014

Keywords

  • ALS
  • Anti-glycolipid antibody
  • CIDP
  • ELISA
  • GBS
  • Non-specific adhesions

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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