A novel dental sealant containing dimethylaminohexadecyl methacrylate suppresses the cariogenic pathogenicity of streptococcus mutans biofilms

Maria Salem Ibrahim, Ahmed S. Ibrahim, Abdulrahman A. Balhaddad, Michael D. Weir, Nancy J. Lin, Franklin R. Tay, Thomas W. Oates, Hockin H.K. Xu, Mary Anne S. Melo

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Cariogenic oral biofilms are strongly linked to dental caries around dental sealants. Quaternary ammonium monomers copolymerized with dental resin systems have been increasingly explored formodulation of biofilmgrowth. Here,we investigated the effect of dimethylaminohexadecyl methacrylate (DMAHDM) on the cariogenic pathogenicity of Streptococcus mutans (S. mutans) biofilms. DMAHDM at 5 mass% was incorporated into a parental formulation containing 20 mass% nanoparticles of amorphous calcium phosphate (NACP). S. mutans biofilms were grown on the formulations, and biofilm inhibition and virulence properties were assessed. The tolerances to acid stress and hydrogen peroxide stress were also evaluated. Our findings suggest that incorporating 5% DMAHDMinto 20% NACP-containing sealants (1) imparts a detrimental biological effect on S. mutans by reducing colony-forming unit counts, metabolic activity and exopolysaccharide synthesis; and (2) reduces overall acid production and tolerance to oxygen stress, two major virulence factors of this microorganism. These results provide a perspective on the value of integrating bioactive restorative materials with traditional caries management approaches in clinical practice. Contact-killing strategies via dental materials aiming to prevent or at least reduce high numbers of cariogenic bacteria may be a promising approach to decrease caries in patients at high risk.

Original languageEnglish (US)
Article number3491
JournalInternational journal of molecular sciences
Volume20
Issue number14
DOIs
StatePublished - Jul 2 2019

Fingerprint

Pit and Fissure Sealants
streptococcus
sealers
biofilms
Streptococcus mutans
Methacrylates
Sealants
Biofilms
Virulence
virulence
calcium phosphates
Calcium phosphate
Nanoparticles
Synthetic Resins
Dental materials
formulations
Dental Materials
nanoparticles
acids
Acids

Keywords

  • Antibacterial agents
  • Biofilm
  • Edental cariesMT
  • Quaternary ammonium compounds
  • Sealant
  • Streptococcus mutans

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

A novel dental sealant containing dimethylaminohexadecyl methacrylate suppresses the cariogenic pathogenicity of streptococcus mutans biofilms. / Ibrahim, Maria Salem; Ibrahim, Ahmed S.; Balhaddad, Abdulrahman A.; Weir, Michael D.; Lin, Nancy J.; Tay, Franklin R.; Oates, Thomas W.; Xu, Hockin H.K.; Melo, Mary Anne S.

In: International journal of molecular sciences, Vol. 20, No. 14, 3491, 02.07.2019.

Research output: Contribution to journalArticle

Ibrahim, Maria Salem ; Ibrahim, Ahmed S. ; Balhaddad, Abdulrahman A. ; Weir, Michael D. ; Lin, Nancy J. ; Tay, Franklin R. ; Oates, Thomas W. ; Xu, Hockin H.K. ; Melo, Mary Anne S. / A novel dental sealant containing dimethylaminohexadecyl methacrylate suppresses the cariogenic pathogenicity of streptococcus mutans biofilms. In: International journal of molecular sciences. 2019 ; Vol. 20, No. 14.
@article{b6934657fe384db280025dd6dcb7dac6,
title = "A novel dental sealant containing dimethylaminohexadecyl methacrylate suppresses the cariogenic pathogenicity of streptococcus mutans biofilms",
abstract = "Cariogenic oral biofilms are strongly linked to dental caries around dental sealants. Quaternary ammonium monomers copolymerized with dental resin systems have been increasingly explored formodulation of biofilmgrowth. Here,we investigated the effect of dimethylaminohexadecyl methacrylate (DMAHDM) on the cariogenic pathogenicity of Streptococcus mutans (S. mutans) biofilms. DMAHDM at 5 mass{\%} was incorporated into a parental formulation containing 20 mass{\%} nanoparticles of amorphous calcium phosphate (NACP). S. mutans biofilms were grown on the formulations, and biofilm inhibition and virulence properties were assessed. The tolerances to acid stress and hydrogen peroxide stress were also evaluated. Our findings suggest that incorporating 5{\%} DMAHDMinto 20{\%} NACP-containing sealants (1) imparts a detrimental biological effect on S. mutans by reducing colony-forming unit counts, metabolic activity and exopolysaccharide synthesis; and (2) reduces overall acid production and tolerance to oxygen stress, two major virulence factors of this microorganism. These results provide a perspective on the value of integrating bioactive restorative materials with traditional caries management approaches in clinical practice. Contact-killing strategies via dental materials aiming to prevent or at least reduce high numbers of cariogenic bacteria may be a promising approach to decrease caries in patients at high risk.",
keywords = "Antibacterial agents, Biofilm, Edental cariesMT, Quaternary ammonium compounds, Sealant, Streptococcus mutans",
author = "Ibrahim, {Maria Salem} and Ibrahim, {Ahmed S.} and Balhaddad, {Abdulrahman A.} and Weir, {Michael D.} and Lin, {Nancy J.} and Tay, {Franklin R.} and Oates, {Thomas W.} and Xu, {Hockin H.K.} and Melo, {Mary Anne S.}",
year = "2019",
month = "7",
day = "2",
doi = "10.3390/ijms20143491",
language = "English (US)",
volume = "20",
journal = "International Journal of Molecular Sciences",
issn = "1661-6596",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "14",

}

TY - JOUR

T1 - A novel dental sealant containing dimethylaminohexadecyl methacrylate suppresses the cariogenic pathogenicity of streptococcus mutans biofilms

AU - Ibrahim, Maria Salem

AU - Ibrahim, Ahmed S.

AU - Balhaddad, Abdulrahman A.

AU - Weir, Michael D.

AU - Lin, Nancy J.

AU - Tay, Franklin R.

AU - Oates, Thomas W.

AU - Xu, Hockin H.K.

AU - Melo, Mary Anne S.

PY - 2019/7/2

Y1 - 2019/7/2

N2 - Cariogenic oral biofilms are strongly linked to dental caries around dental sealants. Quaternary ammonium monomers copolymerized with dental resin systems have been increasingly explored formodulation of biofilmgrowth. Here,we investigated the effect of dimethylaminohexadecyl methacrylate (DMAHDM) on the cariogenic pathogenicity of Streptococcus mutans (S. mutans) biofilms. DMAHDM at 5 mass% was incorporated into a parental formulation containing 20 mass% nanoparticles of amorphous calcium phosphate (NACP). S. mutans biofilms were grown on the formulations, and biofilm inhibition and virulence properties were assessed. The tolerances to acid stress and hydrogen peroxide stress were also evaluated. Our findings suggest that incorporating 5% DMAHDMinto 20% NACP-containing sealants (1) imparts a detrimental biological effect on S. mutans by reducing colony-forming unit counts, metabolic activity and exopolysaccharide synthesis; and (2) reduces overall acid production and tolerance to oxygen stress, two major virulence factors of this microorganism. These results provide a perspective on the value of integrating bioactive restorative materials with traditional caries management approaches in clinical practice. Contact-killing strategies via dental materials aiming to prevent or at least reduce high numbers of cariogenic bacteria may be a promising approach to decrease caries in patients at high risk.

AB - Cariogenic oral biofilms are strongly linked to dental caries around dental sealants. Quaternary ammonium monomers copolymerized with dental resin systems have been increasingly explored formodulation of biofilmgrowth. Here,we investigated the effect of dimethylaminohexadecyl methacrylate (DMAHDM) on the cariogenic pathogenicity of Streptococcus mutans (S. mutans) biofilms. DMAHDM at 5 mass% was incorporated into a parental formulation containing 20 mass% nanoparticles of amorphous calcium phosphate (NACP). S. mutans biofilms were grown on the formulations, and biofilm inhibition and virulence properties were assessed. The tolerances to acid stress and hydrogen peroxide stress were also evaluated. Our findings suggest that incorporating 5% DMAHDMinto 20% NACP-containing sealants (1) imparts a detrimental biological effect on S. mutans by reducing colony-forming unit counts, metabolic activity and exopolysaccharide synthesis; and (2) reduces overall acid production and tolerance to oxygen stress, two major virulence factors of this microorganism. These results provide a perspective on the value of integrating bioactive restorative materials with traditional caries management approaches in clinical practice. Contact-killing strategies via dental materials aiming to prevent or at least reduce high numbers of cariogenic bacteria may be a promising approach to decrease caries in patients at high risk.

KW - Antibacterial agents

KW - Biofilm

KW - Edental cariesMT

KW - Quaternary ammonium compounds

KW - Sealant

KW - Streptococcus mutans

UR - http://www.scopus.com/inward/record.url?scp=85070074318&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85070074318&partnerID=8YFLogxK

U2 - 10.3390/ijms20143491

DO - 10.3390/ijms20143491

M3 - Article

C2 - 31315225

AN - SCOPUS:85070074318

VL - 20

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1661-6596

IS - 14

M1 - 3491

ER -