A novel dynamin-like protein associates with cytoplasmic vesicles and tubules of the endoplasmic reticulum in mammalian cells

Yisang Yoon, Kelly R. Pitts, Sophie Dahan, Mark A. McNiven

Research output: Contribution to journalArticle

137 Citations (Scopus)

Abstract

Dynamins are 100-kilodalton guanosine triphosphatases that participate in the formation of nascent vesicles during endocytosis. Here, we have tested if novel dynamin-like proteins are expressed in mammalian cells to support vesicle trafficking processes at cytoplasmic sites distinct from the plasma membrane. Immunological and molecular biological methods were used to isolate a cDNA clone encoding an 80-kilodalton novel dynamin-like protein, DLP1, that shares up to 42% homology with other dynamin-related proteins. DLP1 is expressed in all tissues examined and contains two alternatively spliced regions that are differentially expressed in a tissue-specific manner. DLP1 is enriched in subcellular membrane fractions of cytoplasmic vesicles and endoplasmic reticulum. Morphological studies of DLP1 in cultured cells using either a specific anti body or an expressed green fluorescent protein (GFP) DLP1 fusion protein revealed that DLPI associates with punctate cytoplasmic vesicles that do not colocalize with conventional dynamin, clathrin, or endocytic ligands. Remarkably, DLP1-positive structures coalign with microtubules and, most strikingly, with endoplasmic reticulum tubules as verified by double labeling with antibodies to calnexin and Rab1 as well as by immunoelectron microscopy. These observations provide the first evidence that a novel dynamin-like protein is expressed in mammalian cells where it associates with a secretory, rather than endocytic membrane compartment.

Original languageEnglish (US)
Pages (from-to)779-793
Number of pages15
JournalJournal of Cell Biology
Volume140
Issue number4
DOIs
StatePublished - Feb 23 1998
Externally publishedYes

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Cytoplasmic Vesicles
Dynamins
Endoplasmic Reticulum
Proteins
Calnexin
Clathrin
Subcellular Fractions
Membranes
Immunoelectron Microscopy
Guanosine
Endocytosis
Green Fluorescent Proteins
Microtubules
Cultured Cells
Complementary DNA
Clone Cells
Cell Membrane
Ligands
Antibodies

ASJC Scopus subject areas

  • Cell Biology

Cite this

A novel dynamin-like protein associates with cytoplasmic vesicles and tubules of the endoplasmic reticulum in mammalian cells. / Yoon, Yisang; Pitts, Kelly R.; Dahan, Sophie; McNiven, Mark A.

In: Journal of Cell Biology, Vol. 140, No. 4, 23.02.1998, p. 779-793.

Research output: Contribution to journalArticle

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