A novel experimental model of erectile dysfunction in rats with heart failure using volume overload

Fábio Henrique Silva, Frederico José Reis Veiga, Aline Gonçalves Mora, Rodrigo Sader Heck, Caroline Candida De Oliveira, Alessandra Gambero, Carla Fernanda Franco-Penteado, Edson Antunes, Jason D. Gardner, Fernanda Priviero, Mário Angelo Claudino

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: Patients with heart failure (HF) display erectile dysfunction (ED). However, the pathophysiology of ED during HF remains poorly investigated. Objective: This study aimed to characterize the aortocaval fistula (ACF) rat model associated with HF as a novel experimental model of ED. We have undertaken molecular and functional studies to evaluate the alterations of the nitric oxide (NO) pathway, autonomic nervous system and oxidative stress in the penis. Methods: Male rats were submitted to ACF for HF induction. Intracavernosal pressure in anesthetized rats was evaluated. Concentration-response curves to contractile (phenylephrine) and relaxant agents (sodium nitroprusside; SNP), as well as to electrical field stimulation (EFS), were obtained in the cavernosal smooth muscle (CSM) strips from sham and HF rats. Protein expression of endothelial NO synthase (eNOS) and neuronal NO synthase (nNOS) and phosphodiestarese-5 in CSM were evaluated, as well as NOX2 (gp91phox) and superoxide dismutase (SOD) mRNA expression. SOD activity and thiobarbituric acid reactive substances (TBARs) were also performed in plasma. Results: HF rats display erectile dysfunction represented by decreased ICP responses compared to sham rats. The neurogenic contractile responses elicited by EFS were greater in CSM from the HF group. Likewise, phenylephrine-induced contractions were greater in CSM from HF rats. Nitrergic response induced by EFS were decreased in the cavernosal tissue, along with lower eNOS, nNOS and phosphodiestarese-5 protein expressions. An increase of NOX2 and SOD mRNA expression in CSM and plasma TBARs of HF group were detected. Plasma SOD activity was decreased in HF rats. Conclusion: ED in HF rats is associated with decreased NO bioavailability in erectile tissue due to eNOS/ nNOS dowregulation and NOX2 upregulation, as well as hypercontractility of the penis. This rat model of ACF could be a useful tool to evaluate the molecular alterations of ED associated with HF.

Original languageEnglish (US)
Article numbere0187083
JournalPloS one
Volume12
Issue number11
DOIs
StatePublished - Nov 1 2017

Fingerprint

Erectile Dysfunction
heart failure
Rats
Theoretical Models
Heart Failure
rats
smooth muscle
Smooth Muscle
Muscle
Nitric Oxide Synthase
Superoxide Dismutase
fistula
superoxide dismutase
electric field
Electric Stimulation
Fistula
phenylephrine
Thiobarbituric Acid Reactive Substances
penis
Penis

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Silva, F. H., Veiga, F. J. R., Mora, A. G., Heck, R. S., De Oliveira, C. C., Gambero, A., ... Claudino, M. A. (2017). A novel experimental model of erectile dysfunction in rats with heart failure using volume overload. PloS one, 12(11), [e0187083]. https://doi.org/10.1371/journal.pone.0187083

A novel experimental model of erectile dysfunction in rats with heart failure using volume overload. / Silva, Fábio Henrique; Veiga, Frederico José Reis; Mora, Aline Gonçalves; Heck, Rodrigo Sader; De Oliveira, Caroline Candida; Gambero, Alessandra; Franco-Penteado, Carla Fernanda; Antunes, Edson; Gardner, Jason D.; Priviero, Fernanda; Claudino, Mário Angelo.

In: PloS one, Vol. 12, No. 11, e0187083, 01.11.2017.

Research output: Contribution to journalArticle

Silva, FH, Veiga, FJR, Mora, AG, Heck, RS, De Oliveira, CC, Gambero, A, Franco-Penteado, CF, Antunes, E, Gardner, JD, Priviero, F & Claudino, MA 2017, 'A novel experimental model of erectile dysfunction in rats with heart failure using volume overload', PloS one, vol. 12, no. 11, e0187083. https://doi.org/10.1371/journal.pone.0187083
Silva FH, Veiga FJR, Mora AG, Heck RS, De Oliveira CC, Gambero A et al. A novel experimental model of erectile dysfunction in rats with heart failure using volume overload. PloS one. 2017 Nov 1;12(11). e0187083. https://doi.org/10.1371/journal.pone.0187083
Silva, Fábio Henrique ; Veiga, Frederico José Reis ; Mora, Aline Gonçalves ; Heck, Rodrigo Sader ; De Oliveira, Caroline Candida ; Gambero, Alessandra ; Franco-Penteado, Carla Fernanda ; Antunes, Edson ; Gardner, Jason D. ; Priviero, Fernanda ; Claudino, Mário Angelo. / A novel experimental model of erectile dysfunction in rats with heart failure using volume overload. In: PloS one. 2017 ; Vol. 12, No. 11.
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abstract = "Background: Patients with heart failure (HF) display erectile dysfunction (ED). However, the pathophysiology of ED during HF remains poorly investigated. Objective: This study aimed to characterize the aortocaval fistula (ACF) rat model associated with HF as a novel experimental model of ED. We have undertaken molecular and functional studies to evaluate the alterations of the nitric oxide (NO) pathway, autonomic nervous system and oxidative stress in the penis. Methods: Male rats were submitted to ACF for HF induction. Intracavernosal pressure in anesthetized rats was evaluated. Concentration-response curves to contractile (phenylephrine) and relaxant agents (sodium nitroprusside; SNP), as well as to electrical field stimulation (EFS), were obtained in the cavernosal smooth muscle (CSM) strips from sham and HF rats. Protein expression of endothelial NO synthase (eNOS) and neuronal NO synthase (nNOS) and phosphodiestarese-5 in CSM were evaluated, as well as NOX2 (gp91phox) and superoxide dismutase (SOD) mRNA expression. SOD activity and thiobarbituric acid reactive substances (TBARs) were also performed in plasma. Results: HF rats display erectile dysfunction represented by decreased ICP responses compared to sham rats. The neurogenic contractile responses elicited by EFS were greater in CSM from the HF group. Likewise, phenylephrine-induced contractions were greater in CSM from HF rats. Nitrergic response induced by EFS were decreased in the cavernosal tissue, along with lower eNOS, nNOS and phosphodiestarese-5 protein expressions. An increase of NOX2 and SOD mRNA expression in CSM and plasma TBARs of HF group were detected. Plasma SOD activity was decreased in HF rats. Conclusion: ED in HF rats is associated with decreased NO bioavailability in erectile tissue due to eNOS/ nNOS dowregulation and NOX2 upregulation, as well as hypercontractility of the penis. This rat model of ACF could be a useful tool to evaluate the molecular alterations of ED associated with HF.",
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AU - Veiga, Frederico José Reis

AU - Mora, Aline Gonçalves

AU - Heck, Rodrigo Sader

AU - De Oliveira, Caroline Candida

AU - Gambero, Alessandra

AU - Franco-Penteado, Carla Fernanda

AU - Antunes, Edson

AU - Gardner, Jason D.

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AU - Claudino, Mário Angelo

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N2 - Background: Patients with heart failure (HF) display erectile dysfunction (ED). However, the pathophysiology of ED during HF remains poorly investigated. Objective: This study aimed to characterize the aortocaval fistula (ACF) rat model associated with HF as a novel experimental model of ED. We have undertaken molecular and functional studies to evaluate the alterations of the nitric oxide (NO) pathway, autonomic nervous system and oxidative stress in the penis. Methods: Male rats were submitted to ACF for HF induction. Intracavernosal pressure in anesthetized rats was evaluated. Concentration-response curves to contractile (phenylephrine) and relaxant agents (sodium nitroprusside; SNP), as well as to electrical field stimulation (EFS), were obtained in the cavernosal smooth muscle (CSM) strips from sham and HF rats. Protein expression of endothelial NO synthase (eNOS) and neuronal NO synthase (nNOS) and phosphodiestarese-5 in CSM were evaluated, as well as NOX2 (gp91phox) and superoxide dismutase (SOD) mRNA expression. SOD activity and thiobarbituric acid reactive substances (TBARs) were also performed in plasma. Results: HF rats display erectile dysfunction represented by decreased ICP responses compared to sham rats. The neurogenic contractile responses elicited by EFS were greater in CSM from the HF group. Likewise, phenylephrine-induced contractions were greater in CSM from HF rats. Nitrergic response induced by EFS were decreased in the cavernosal tissue, along with lower eNOS, nNOS and phosphodiestarese-5 protein expressions. An increase of NOX2 and SOD mRNA expression in CSM and plasma TBARs of HF group were detected. Plasma SOD activity was decreased in HF rats. Conclusion: ED in HF rats is associated with decreased NO bioavailability in erectile tissue due to eNOS/ nNOS dowregulation and NOX2 upregulation, as well as hypercontractility of the penis. This rat model of ACF could be a useful tool to evaluate the molecular alterations of ED associated with HF.

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